PMID- 15342934
OWN - NLM
STAT- MEDLINE
DCOM- 20050325
LR  - 20220321
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 22
IP  - 5
DP  - 2004
TI  - Elevation of platelet activation markers and chemokines during peripheral blood 
      stem cell harvest with G-CSF.
PG  - 696-703
AB  - The kinetics of peripheral blood stem cell mobilization in response to 
      recombinant human granulocyte colony-stimulating factor is well established. 
      However, there have been few investigations of platelet activation markers during 
      peripheral blood stem cell harvest. We measured the levels of the platelet 
      activation markers, chemokines, and soluble factors in plasma obtained from 
      patients undergoing peripheral blood stem cell harvest. The number of leukocytes, 
      CD34+ cells, neutrophils, monocytes, and lymphocytes peaked on day 5 after 
      granulocyte colony-stimulating factor treatment, but the numbers of eosinophils 
      and basophils showed no significant change. Regulated on activation normally 
      T-cell expressed and secreted (RANTES) level increased through day 10, and the 
      monocyte chemotactic peptide-1 (MCP-1) level peaked on day 5. Platelet counts 
      continued to increase through day 10. The level of thrombopoietin significantly 
      increased on day 3, peaked on day 5, and decreased slightly by day 10. The levels 
      of soluble CD40 ligand and soluble P-selectin increased up to day 5. The 
      platelet-derived microparticle level peaked on day 5, and then began to decline. 
      CD34+ cell numbers significantly correlated with those of leucocytes, 
      neutrophils, monocytes, and lymphocytes, as well as levels of MCP-1, and the 
      CD34+ cells exhibited changes similar to platelet-derived microparticles. The 
      patterns of change in MCP-1, platelet-derived microparticles, and the CD34+ cell 
      count are similar in that each peaks on day 5 and decreases thereafter. Further 
      study is required to determine if a cause-and-effect relationship in their 
      pattern of change exists among them.
FAU - Nomura, Shosaku
AU  - Nomura S
AD  - First Department of Internal Medicine, Kansai Medical University, 10-15 
      Fumizono-cho, Moriguchi, Osaka 570-8507, Japan. shosaku-n@mbp.ocn.ne.jp
FAU - Inami, Norihito
AU  - Inami N
FAU - Kanazawa, Shigenori
AU  - Kanazawa S
FAU - Iwasaka, Toshiji
AU  - Iwasaka T
FAU - Fukuhara, Shirou
AU  - Fukuhara S
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Antigens, CD34)
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines)
RN  - 0 (P-Selectin)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 9014-42-0 (Thrombopoietin)
SB  - IM
MH  - Adult
MH  - Antigens, CD34/drug effects/immunology
MH  - Biomarkers/blood
MH  - CD40 Ligand/blood
MH  - Cell Proliferation/drug effects
MH  - Chemokine CCL2/blood/immunology
MH  - Chemokine CCL5/blood/immunology
MH  - Chemokines/*blood/immunology
MH  - Female
MH  - Granulocyte Colony-Stimulating Factor/*pharmacology/therapeutic use
MH  - Granulocytes/drug effects/immunology
MH  - Hematopoietic Stem Cell Mobilization/*methods
MH  - Humans
MH  - Leukapheresis/*methods
MH  - Lymphocytes/drug effects/immunology
MH  - Male
MH  - Middle Aged
MH  - Monocytes/drug effects/immunology
MH  - P-Selectin/blood
MH  - Platelet Activation/drug effects/*immunology
MH  - Platelet Count
MH  - Thrombopoietin/blood
MH  - Up-Regulation/drug effects/immunology
EDAT- 2004/09/03 05:00
MHDA- 2005/03/26 09:00
CRDT- 2004/09/03 05:00
PHST- 2004/09/03 05:00 [pubmed]
PHST- 2005/03/26 09:00 [medline]
PHST- 2004/09/03 05:00 [entrez]
AID - 22/5/696 [pii]
AID - 10.1634/stemcells.22-5-696 [doi]
PST - ppublish
SO  - Stem Cells. 2004;22(5):696-703. doi: 10.1634/stemcells.22-5-696.