PMID- 15344932
OWN - NLM
STAT- MEDLINE
DCOM- 20041109
LR  - 20171116
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 6
IP  - 10
DP  - 2004 Oct
TI  - Impact of lime, nitrogen and plant species on bacterial community structure in 
      grassland microcosms.
PG  - 1070-80
AB  - A microcosm-based approach was used to study impacts of plant and chemical 
      factors on the bacterial community structure of an upland acidic grassland soil. 
      Seven perennial plant species typical of both natural, unimproved (Nardus 
      stricta, Agrostis capillaris, Festuca ovina and F. rubra) and fertilized, 
      improved (Holcus lanatus, Lolium perenne and Trifolium repens) grasslands were 
      either left unamended or treated with lime, nitrogen, or lime plus nitrogen in a 
      75-day glasshouse experiment. Lime and nitrogen amendment were shown to have a 
      greater effect on microbial activity, biomass and bacterial ribotype number than 
      plant species. Liming increased soil pH, microbial activity and biomass, while 
      decreasing ribotype number. Nitrogen addition decreased soil pH, microbial 
      activity and ribotype number. Addition of lime plus nitrogen had intermediate 
      effects, which appeared to be driven more by lime than nitrogen. Terminal 
      restriction fragment length polymorphism (TRFLP) analysis revealed that lime and 
      nitrogen addition altered soil bacterial community structure, while plant species 
      had little effect. These results were further confirmed by multivariate 
      redundancy analysis, and suggest that soil lime and nitrogen status are more 
      important controllers of bacterial community structure than plant rhizosphere 
      effects.
FAU - Kennedy, Nabla
AU  - Kennedy N
AD  - Microbial Ecology Group, Department of Industrial Microbiology, University 
      College Dublin, Belfield, Dublin 4, Ireland.
FAU - Brodie, Eoin
AU  - Brodie E
FAU - Connolly, John
AU  - Connolly J
FAU - Clipson, Nicholas
AU  - Clipson N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Calcium Compounds)
RN  - 0 (DNA Primers)
RN  - 0 (Oxides)
RN  - 0 (Soil)
RN  - C7X2M0VVNH (lime)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Analysis of Variance
MH  - Bacteria/*drug effects/genetics
MH  - *Bacterial Physiological Phenomena
MH  - Biomass
MH  - Calcium Compounds/*pharmacology
MH  - DNA Primers
MH  - *Ecosystem
MH  - Hydrogen-Ion Concentration
MH  - Multivariate Analysis
MH  - Nitrogen/*pharmacology
MH  - Oxides/*pharmacology
MH  - Poaceae/physiology
MH  - Polymorphism, Restriction Fragment Length
MH  - Soil/analysis
EDAT- 2004/09/04 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/09/04 05:00
PHST- 2004/09/04 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/09/04 05:00 [entrez]
AID - EMI638 [pii]
AID - 10.1111/j.1462-2920.2004.00638.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2004 Oct;6(10):1070-80. doi: 10.1111/j.1462-2920.2004.00638.x.