PMID- 15344953
OWN - NLM
STAT- MEDLINE
DCOM- 20041210
LR  - 20071115
IS  - 0902-0063 (Print)
IS  - 0902-0063 (Linking)
VI  - 18
IP  - 5
DP  - 2004 Oct
TI  - Effect of combined T- and B-cell depletion of allogeneic HLA-mismatched bone 
      marrow graft on the magnitude and kinetics of Epstein-Barr virus load in the 
      peripheral blood of bone marrow transplant recipients.
PG  - 518-24
AB  - Recipients of T-cell-depleted bone marrow (BM) transplants (BMT) frequently 
      develop Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative 
      disease (PTLD) preceded by a rapid and prominent increase of EBV load in the 
      peripheral blood. The level of this increase positively correlates with the 
      incidence of PTLD. Using a semiquantitative PCR assay we compared the blood 
      levels of EBV-DNA in patients transplanted with either T-cell or T- and 
      B-cell-depleted human leukocyte antigen (HLA)-mismatched BM grafts. Combined T- 
      and B-cell depletion correlated with significantly lower maximal levels of EBV 
      load, which were reached with slower kinetics. These data indicate that B-cell 
      depletion of BM can be used for prophylaxis of PTLD in BM transplant recipients 
      and can affect the long-term balance between EBV and its host.
FAU - Liu, Daorong
AU  - Liu D
AD  - Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden.
FAU - Tammik, Charlotte
AU  - Tammik C
FAU - Zou, Jie-Zhi
AU  - Zou JZ
FAU - Ernberg, Ingemar
AU  - Ernberg I
FAU - Masucci, Maria G
AU  - Masucci MG
FAU - Ringden, Olle
AU  - Ringden O
FAU - Levitsky, Victor
AU  - Levitsky V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Clin Transplant
JT  - Clinical transplantation
JID - 8710240
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - B-Lymphocytes/*pathology
MH  - Bone Marrow Transplantation/*pathology
MH  - Child, Preschool
MH  - Epstein-Barr Virus Infections/prevention & control
MH  - Female
MH  - Follow-Up Studies
MH  - HLA Antigens/*immunology
MH  - Herpesvirus 4, Human/isolation & purification/*physiology
MH  - Histocompatibility/*immunology
MH  - Humans
MH  - Leukemia, Myeloid/therapy
MH  - Leukocyte Reduction Procedures/*methods
MH  - Male
MH  - Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/therapy
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/therapy
MH  - T-Lymphocytes/*pathology
MH  - Transplantation Conditioning
MH  - Transplantation, Homologous
MH  - *Viral Load
MH  - Viremia/*virology
EDAT- 2004/09/04 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/09/04 05:00
PHST- 2004/09/04 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/09/04 05:00 [entrez]
AID - CTR198 [pii]
AID - 10.1111/j.1399-0012.2004.00198.x [doi]
PST - ppublish
SO  - Clin Transplant. 2004 Oct;18(5):518-24. doi: 10.1111/j.1399-0012.2004.00198.x.