PMID- 15347317
OWN - NLM
STAT- MEDLINE
DCOM- 20041208
LR  - 20171116
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 73
IP  - 4
DP  - 2004 Oct
TI  - Flow-cytometric approach to the prompt laboratory diagnosis of TRALI: a case 
      report.
PG  - 295-9
AB  - OBJECTIVES: Transfusion-related acute lung injury (TRALI) is a rare but serious 
      complication which can occur after transfusion of blood components. In this 
      report we describe our flow-cytometry approach to the laboratory diagnosis of a 
      case of TRALI in a recipient of fresh frozen plasma containing human leukocyte 
      antigen (HLA) class II antibodies. METHODS: The post-transfusion reaction work-up 
      included the direct and indirect Granulocyte Immunofluorescence Test (GIFT) on 
      the recipient's neutrophils collected before and after the reaction and on the 
      serum from the recipient and from all implicated donors; flow-cytometry 
      bead-based screening and identification assay for HLA class I and II antibodies 
      in donor sera and flow cytometry cross-matching on T and B patient's lymphocytes. 
      Finally, we investigated the reactivity of one donor serum, containing HLA class 
      II antibodies, with the patient's neutrophils activated in vitro to induce 
      expression of HLA class II. RESULTS: We found an increased level of IgG bound on 
      patient's granulocytes collected after TRALI, in the absence of detectable 
      granulocyte and HLA class I antibodies in the five implicated donors. One of them 
      showed HLA-DR 1 and -DR 51 antibodies, which determined a positive cross-match 
      with patient's B lymphocytes and in vitro activated granulocytes. Both HLA class 
      II antigens were present in the recipient and absent in the donor. CONCLUSIONS: 
      In some pathological conditions, HLA class II antibodies can react with activated 
      granulocytes expressing HLA-DR antigens, and activate TRALI reaction. HLA class 
      II antibodies screening and flow cytometry cross-matching techniques should be 
      added to the current diagnostic algorithm of TRALI.
CI  - Copyright Blackwell Munksgaard 2004.
FAU - Porretti, Laura
AU  - Porretti L
AD  - Servizio Autonomo per il Prelievo e Trapianto di Organi e Tessuti, IRCCS Ospedale 
      Maggiore Policlinico, Milan, Italy. lauraporr@libero.it
FAU - Coluccio, Elena
AU  - Coluccio E
FAU - Prati, Daniele
AU  - Prati D
FAU - Colombo, Maria B
AU  - Colombo MB
FAU - Lopa, Raffaella
AU  - Lopa R
FAU - Tombolini, Pietro
AU  - Tombolini P
FAU - Ambrosone, Antonella
AU  - Ambrosone A
FAU - Crespiatico, Loretta
AU  - Crespiatico L
FAU - Scalamogna, Mario
AU  - Scalamogna M
FAU - Rebulla, Paolo
AU  - Rebulla P
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
SB  - IM
MH  - Aged
MH  - Flow Cytometry
MH  - Humans
MH  - *Lung Injury
MH  - Male
MH  - *Transfusion Reaction
EDAT- 2004/09/07 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/09/07 05:00
PHST- 2004/09/07 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/09/07 05:00 [entrez]
AID - EJH279 [pii]
AID - 10.1111/j.1600-0609.2004.00279.x [doi]
PST - ppublish
SO  - Eur J Haematol. 2004 Oct;73(4):295-9. doi: 10.1111/j.1600-0609.2004.00279.x.