PMID- 1534784
OWN - NLM
STAT- MEDLINE
DCOM- 19920715
LR  - 20130418
IS  - 0971-5916 (Print)
IS  - 0971-5916 (Linking)
VI  - 95
DP  - 1992 Mar
TI  - Variations in isoenzymes of cloned & uncloned axenic Entamoeba histolytica 
      without bacterial association.
PG  - 88-92
AB  - Five clones of axenic E. histolytica (HMI) grown as discrete colonies in 
      semisolid agar medium were adapted in liquid medium and labelled as HMI-C121, 
      HMI-C131, HMI-C143, HMI-C144 and HMI-C145. Isoenzymes of these 5 clones of E. 
      histolytica (HMI) were investigated in starch gel electrophoresis. There were no 
      differences in the electromobility of maleate NADP oxidoreductase and 
      glucosephosphoisomerase amongst the five clones and uncloned cultures of axenic 
      E. histolytica. The relative electromobility (rf) of a single phosphoglucomutase 
      (PGM) band of uncloned Mexican E. histolytica (HMI) and Indian axenic E. 
      histolytica (KCG: 0986: 11) cultures and cloned E. histolytica HMI-C121, HMI-C145 
      was 0.087 while a single PGM band of uncloned E. histolytica (NIH: 200) and 
      cloned E. histolytica HMI-C131, HMI-C143 and HMI-C144 cultures had rf of 0.075. 
      Isoenzyme characterization of four cloned HMI-C121, HMI-C131, HMI-C143, HMI-C144 
      cultures of axenic E. histolytica (HMI) revealed existence of three bands of 
      hexokinase (HK). The additional third band of HK was located close to the place 
      of application of lysate and had rf ranging from 0.11-0.14. The data indicated 
      that parent axenic E. histolytica (HMI) consisted of several populations and each 
      population expressed different isoenzyme pattern without an association of 
      amoebic cultures with any bacterial species.
FAU - Romana, S
AU  - Romana S
AD  - Department of Experimental Medicine, Postgraduate Institute of Medical Education 
      & Research Chandigarh.
FAU - Vinayak, V K
AU  - Vinayak VK
LA  - eng
PT  - Journal Article
PL  - India
TA  - Indian J Med Res
JT  - The Indian journal of medical research
JID - 0374701
RN  - 0 (Isoenzymes)
RN  - EC 1.6.- (NADH, NADPH Oxidoreductases)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
RN  - EC 5.4.2.2 (Phosphoglucomutase)
SB  - IM
MH  - Animals
MH  - Clone Cells
MH  - Entamoeba histolytica/*enzymology
MH  - Glucose-6-Phosphate Isomerase/analysis
MH  - Hexokinase/analysis
MH  - Isoenzymes/*analysis
MH  - NADH, NADPH Oxidoreductases/analysis
MH  - Phosphoglucomutase/analysis
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
PST - ppublish
SO  - Indian J Med Res. 1992 Mar;95:88-92.