PMID- 15347887
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1523-7834 (Print)
IS  - 1523-7834 (Linking)
VI  - 30
IP  - 3
DP  - 2004
TI  - Utility of Interphase FISH Panels for Routine Clinical Cytogenetic Evaluation of 
      Chronic Lymphocytic Leukemia and Multiple Myeloma.
PG  - 77-81
AB  - Specific genetic abnormalities are of prognostic significance for patients with 
      chronic lymphocytic leukemia (CLL) and multiple myeloma (MM); however, routine 
      cytogenetic analysis usually provides normal results. We utilized two probe 
      panels for interphase fluorescence in situ hybridization (FISH) studies to 
      enhance the ability to detect genetic abnormalities in samples that were referred 
      for routine cytogenetic studies for possible diagnoses of CLL or MM. The CLL 
      panel consisted of probes for 11q22.3 (ATM gene), 13q14 (D13S319), the centromere 
      of chromosome 12 (D12Z3) and 17p13.1 (P53 gene). The MM panel included probes for 
      14q32 (IgH gene) and/or t(11:14)(q13;q32) (BCL1/IgH), 13q14 (D13S319) and 17p13.1 
      (P53 gene). FISH detected clonal aberrations not identified by conventional 
      cytogenetics in an additional 8 of 23 (35%) samples referred for possible CLL and 
      7 of 42 (17%) samples with possible MM. The prognostic significance of the 
      aberrations identified ranged from favorable, to intermediate, to poor. Our 
      studies indicate that many samples referred for routine cytogenetics testing for 
      CLL and MM yield normal results for both conventional and FISH testing, likely 
      due to lack of definitive diagnosis in a percentage of cases. However, FISH is 
      more sensitive for the detection of clinically significant chromosome 
      abnormalities and should be the testing methodology of choice for these 
      disorders.
FAU - Cady, Francois M
AU  - Cady FM
AD  - Department of Pathology and Medical University of South Carolina (MUSC) 
      Laboratory Medicine, 165 Ashley Avenue, Suite 309, Charleston, SC 29425. 
      wolffd@musc.edu.
FAU - Muto, Deidra N
AU  - Muto DN
FAU - Ciabeterri, Gena
AU  - Ciabeterri G
FAU - Johns, Alan
AU  - Johns A
FAU - Gainey Church, Keisha
AU  - Gainey Church K
FAU - Wolff, Daynna J
AU  - Wolff DJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Assoc Genet Technol
JT  - Journal of the Association of Genetic Technologists
JID - 9807282
EDAT- 2004/09/07 05:00
MHDA- 2004/09/07 05:01
CRDT- 2004/09/07 05:00
PHST- 2004/09/07 05:00 [pubmed]
PHST- 2004/09/07 05:01 [medline]
PHST- 2004/09/07 05:00 [entrez]
PST - ppublish
SO  - J Assoc Genet Technol. 2004;30(3):77-81.