PMID- 15348064
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20050307
LR  - 20191026
IS  - 0957-4530 (Print)
IS  - 0957-4530 (Linking)
VI  - 11
IP  - 12
DP  - 2000 Dec
TI  - Surface topography and HA filler volume effect on primary human osteoblasts in 
      vitro.
PG  - 805-10
AB  - HAPEX, a bone analog material, with similar properties to cortical bone, has been 
      studied in vitro with particular reference to the effect of surface topography. 
      The stimulation of a favorable bone response by this composite depends on 
      optimization of the hydroxyapatite (HA) content in relation to the material 
      bioactivity without compromising the mechanical characteristics. In this study we 
      have started to investigate the effects of surface topography on cell attachment 
      and subsequent cellular behavior in relation to proliferation. Different volumes 
      of HA (20% and 40%) were added to a high density polyethylene (HDPE) matrix to 
      produce the test materials. Scanning electron microscopy (SEM) and confocal laser 
      scanning microscopy (CLSM) were used to examine cell morphology on HAPEX, and the 
      surface characteristics produced by different machining protocols. The 
      measurement of cellular DNA and tritiated thymidine ([3H]-TdR) incorporation has 
      been used to asses cell proliferation upon the materials. The results show that 
      the material surface topography has a large influence on cell proliferation and 
      attachment, and with a controlled material topography the 40% vol HA/HDPE 
      composite gives the greater biological response compared to the 20% vol HA/HDPE 
      composite.
CI  - Copyright 2000 Kluwer Academic Publishers
FAU - Dalby, M J
AU  - Dalby MJ
AD  - IRC in Biomedical Materials, Institute of Orthopaedics, Brockley Hill, Stanmore, 
      Middlesex, HA7 4LP, UK. M.J.Dalby@qmw.ac.uk
FAU - Di Silvio, L
AU  - Di Silvio L
FAU - Davies, G W
AU  - Davies GW
FAU - Bonfield, W
AU  - Bonfield W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
EDAT- 2004/09/07 05:00
MHDA- 2004/09/07 05:01
CRDT- 2004/09/07 05:00
PHST- 2004/09/07 05:00 [pubmed]
PHST- 2004/09/07 05:01 [medline]
PHST- 2004/09/07 05:00 [entrez]
AID - 281652 [pii]
AID - 10.1023/a:1008957630020 [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2000 Dec;11(12):805-10. doi: 10.1023/a:1008957630020.