PMID- 15348951
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20050307
LR  - 20191026
IS  - 0957-4530 (Print)
IS  - 0957-4530 (Linking)
VI  - 9
IP  - 12
DP  - 1998 Dec
TI  - In vitro response of osteoblasts to hydroxyapatite-reinforced polyethylene 
      composites.
PG  - 845-8
AB  - A primary human cell culture model was used to investigate a range of 
      hydroxyapaptite (HA)-reinforced high-density polyethylene (HDPE) composites 
      (HAPEX). These materials are being developed as potential bone-substitute 
      materials. When designing and optimizing a second-generation biomaterial, it is 
      important to achieve a balance between mechanical and biological properties 
      without compromising either. Biochemical and histological parameters have been 
      used to compare the biological response of 20% and 40% volume HA in HDPE. 
      Cellular DNA and incorporation of tritiated thymidine was measured to assess cell 
      proliferation. Alkaline phosphatase (ALP) production was used as a marker of 
      osteoblast phenotype expression. In this preliminary study, osteoblasts cultured 
      on the 20% HAPEX showed a greater increase in the rate of proliferation and 
      osteoblast expression as indicated by an increase in ALP activity compared to the 
      40% HAPEX over the time period studied. Osteoblast-like cells showed a flattened 
      morphology on both composites and in some cases a greater covering was observed 
      on the 20% HAPEX. These results indicate that the composites may not be identical 
      in terms of bioactivity and that further research on surface topography and 
      physico-chemical properties is required to assess fully the biological response 
      of these composites.
CI  - Copyright 1998 Kluwer Academic Publishers
FAU - Di Silvio, L
AU  - Di Silvio L
AD  - IRC in Biomedical Materials, Institute of Orthopaedics (UCL), Brockley Hill, 
      Stanmore, Middlesex HA7 4LP, UK. ldisilvio@ucl.ac.uk
FAU - Dalby, M
AU  - Dalby M
FAU - Bonfield, W
AU  - Bonfield W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
EDAT- 2004/09/07 05:00
MHDA- 2004/09/07 05:01
CRDT- 2004/09/07 05:00
PHST- 2004/09/07 05:00 [pubmed]
PHST- 2004/09/07 05:01 [medline]
PHST- 2004/09/07 05:00 [entrez]
AID - 230476 [pii]
AID - 10.1023/a:1008900312950 [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 1998 Dec;9(12):845-8. doi: 10.1023/a:1008900312950.