PMID- 15349665
OWN - NLM
STAT- MEDLINE
DCOM- 20061120
LR  - 20130405
IS  - 1006-7248 (Print)
IS  - 1006-7248 (Linking)
VI  - 13
IP  - 4
DP  - 2004 Aug
TI  - [Bone formation by seeding bone marrow stromal cells on the sodium calcium 
      phosphate/beta tricalcium phosphate scaffold].
PG  - 278-81
AB  - PURPOSE: To observe the osteogenesis of sodium calcium phosphate/beta-tricalcium 
      phosphate scaffold loaded with marrow stromal cells in a nude mice subcutaneous 
      model. METHODS: To transform the sintered bovine cancellous bone into 
      NaCaPO(4)/beta-TCP biphasic calcium phosphate ceramic by physical and chemical 
      methods. Bone marrow stromal cells (BMSCs) were obtained by harvesting the 
      cancellous bone from the iliac crest of a rabbit. Then the BMSCs were isolated, 
      expanded and induced in vitro and seeded in the NaCaPO(4)/beta-TCP ceramic 
      scaffolds. The scaffold/BMSCs composites were implanted subcutaneously into the 
      backs of nude mice. Implantation of NaCaPO(4)/beta-TCP ceramic alone was acted as 
      control. At 4 and 8 weeks after implantation, the specimens were harvested and 
      the osteogenetic activity were evaluated by gross and histologic observation. 
      RESULTS: At 4 weeks after implantation, the relatively mature bone were seen on 
      the surface of the material, the inner part of the material were mainly 
      cartilaginous bone. At 8 weeks after implantation, A large amount of trabecula 
      bone had formed and the medullary cavity, medullary cells and lipocytes could be 
      seen. Osteoblasts and osteoclasts were seen between the scaffolds and the local 
      bone in the transition area. The endochondral bone formation pattern were 
      observed. CONCLUSIONS: The NaCaPO(4)/beta-TCP scaffold/BMSCs composites showed 
      good osteogenetic activity and could promote mineralization of the immature bone. 
      It can be used as the bone tissue engineering scaffolds.
FAU - Yang, Yao-Wu
AU  - Yang YW
AD  - Department of Oral and Maxillofacial Surgery, Stomatological College, Fourth 
      Military Medical University, Xi'an 710032, Shanxi Province, China. 
      yangyaowu@fm-mu.edu.cn
FAU - Lei, De-Lin
AU  - Lei DL
FAU - Mao, Tian-Qiu
AU  - Mao TQ
FAU - Hou, Rui
AU  - Hou R
FAU - Li, Jian-Hu
AU  - Li JH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Shanghai Kou Qiang Yi Xue
JT  - Shanghai kou qiang yi xue = Shanghai journal of stomatology
JID - 101090220
RN  - 0 (Calcium Phosphates)
RN  - 0 (beta-tricalcium phosphate)
RN  - 97Z1WI3NDX (calcium phosphate)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Calcium Phosphates/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Osteoblasts/*cytology
MH  - Rabbits
MH  - Stromal Cells/*cytology
MH  - Tissue Engineering/methods
EDAT- 2004/09/07 05:00
MHDA- 2006/12/09 09:00
CRDT- 2004/09/07 05:00
PHST- 2004/09/07 05:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2004/09/07 05:00 [entrez]
AID - 1558 [pii]
PST - ppublish
SO  - Shanghai Kou Qiang Yi Xue. 2004 Aug;13(4):278-81.