PMID- 15349938
OWN - NLM
STAT- MEDLINE
DCOM- 20050307
LR  - 20230120
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 25
IP  - 17
DP  - 2004 Sep
TI  - Determination of amino acids in rat vitreous perfusates by capillary 
      electrophoresis.
PG  - 2978-84
AB  - In vivo determinations of amino acids are important for improving our 
      understanding of physiological states of biological tissue function and 
      dysfunction. However, the chemically complex matrix of different biological 
      fluids complicates the assay of this important class of molecules. We introduce a 
      method for characterizing the amino acid composition of submicroliter volumes of 
      vitreous humor perfusates. Low-flow push-pull perfusion sampling is compatible 
      with collecting small volume samples in a complicated matrix that are potentially 
      difficult to separate. An efficient, sensitive, and rapid analysis of amino acids 
      from in vivo perfusates of the vitreous is presented with 
      3-(4-carboxybenzoyl)-2-quinoline-carboxaldehyde (CBQCA) derivatitation and 
      capillary electrophoresis (CE) separation with laser-induced fluorescence 
      detection (LIF). Derivatization with CBQCA for up to 2 h provided high 
      sensitivity and low detection limits at the nM level. Seventeen amino acids 
      including D-serine (D-Ser) and D-aspartate (D-Asp) were resolved in less than 10 
      min. Importantly, D-Ser is separated from its enantiomeric pair. Characterization 
      of vitreal amino acids with this assay technique will be useful for understanding 
      ocular diseases and physiological mechanisms in vision.
FAU - Thongkhao-On, Kongthong
AU  - Thongkhao-On K
AD  - Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, 
      USA.
FAU - Kottegoda, Sumith
AU  - Kottegoda S
FAU - Pulido, Jose S
AU  - Pulido JS
FAU - Shippy, Scott A
AU  - Shippy SA
LA  - eng
GR  - EY014908/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Amino Acids)
RN  - 0 (Indicators and Reagents)
SB  - IM
MH  - Amino Acids/*analysis/isolation & purification
MH  - Animals
MH  - Electrophoresis, Capillary/methods
MH  - Indicators and Reagents
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Vitreous Body/*chemistry
EDAT- 2004/09/07 05:00
MHDA- 2005/03/08 09:00
CRDT- 2004/09/07 05:00
PHST- 2004/09/07 05:00 [pubmed]
PHST- 2005/03/08 09:00 [medline]
PHST- 2004/09/07 05:00 [entrez]
AID - 10.1002/elps.200405941 [doi]
PST - ppublish
SO  - Electrophoresis. 2004 Sep;25(17):2978-84. doi: 10.1002/elps.200405941.