PMID- 15350300
OWN - NLM
STAT- MEDLINE
DCOM- 20041207
LR  - 20071115
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 153
IP  - 2
DP  - 2004 Sep
TI  - Cytogenetics and fluorescence in situ hybridization studies of diffuse large 
      B-cell lymphoma in children and young adults.
PG  - 115-21
AB  - Diffuse large B-cell lymphoma (DLBCL), the most common subtype of adult 
      non-Hodgkin lymphoma (NHL), is infrequently seen in adolescents and is rare in 
      children. Due to the infrequency of the disease, single institution-based 
      cytogenetic and fluorescence in situ hybridization (FISH) studies of pediatric 
      DLBCL have not been reported so far and, hence, the possible differences in 
      pediatric and adult DLBCL have not been evaluated. We performed cytogenetic and 
      FISH analyses of 7 pediatric and 5 young adult DLBCL cases referred to the 
      University of Nebraska Medical Center. Karyotypic studies revealed numeric and 
      structural chromosome abnormalities in all cases. Loss of chromosomes 2, 3, 4, 6, 
      12, 15, 16, and 17, and gain of 12, 18, and X were observed in more than 20% of 
      the cases (#10878;3 cases). Sex chromosome abnormalities and cytogenetically 
      unidentifiable chromosomes and/or segments were observed in 80% (10/12) of the 
      cases. Recurrent breakpoints (observed in 3 or more cases) included 14q32 (IGH) 
      and 17p13 (TP53), which clustered in the young adult group. The breakpoints 7q36, 
      9p24, 13q34, and 16q24 were noted in two cases each. We performed interphase FISH 
      studies to verify the possible rearrangements of the breakpoints that are 
      frequently implicated in adult DLBCL. Our results confirmed that the pediatric 
      cases did not show rearrangements of 3q27 (BCL6), 14q32 (IGH), 18q21 (BCL2), 8q24 
      (CMYC), and 17p13 (TP53), except for one case with IGH;BCL2 dual fusion 
      [t(14;18)(q32;q21)] and one with a 17p13 (TP53) deletion. Although 3q27 was noted 
      to be rearranged by conventional cytogenetics in two young adult DLBCL cases, 
      FISH investigations verified that BCL6 was not disrupted. The t(8;14)(q24;q32) 
      with rearranged CMYC ascertained by FISH, was observed in a single young adult 
      DLBCL case. These results highlight a distinctly different representation of 
      cytogenetic abnormalities in pediatric versus adult DLBCL.
FAU - Dave, Bhavana J
AU  - Dave BJ
AD  - Human Genetics Laboratory, Munroe Meyer Institute for Genetics and 
      Rehabilitation, 985440 Nebraska Medical Center, Omaha, NE 68198-5440, USA. 
      bdave@unmc.edu
FAU - Weisenburger, Dennis D
AU  - Weisenburger DD
FAU - Higgins, Christine M
AU  - Higgins CM
FAU - Pickering, Diane L
AU  - Pickering DL
FAU - Hess, Michelle M
AU  - Hess MM
FAU - Chan, Wing C
AU  - Chan WC
FAU - Sanger, Warren G
AU  - Sanger WG
LA  - eng
GR  - CA36727/CA/NCI NIH HHS/United States
GR  - U01 CA84967/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Chromosome Aberrations
MH  - Chromosome Mapping
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lymphoma, B-Cell/*genetics/pathology
MH  - Lymphoma, Large B-Cell, Diffuse/*genetics/pathology
MH  - Male
EDAT- 2004/09/08 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/09/08 05:00
PHST- 2003/11/05 00:00 [received]
PHST- 2004/01/06 00:00 [revised]
PHST- 2004/01/08 00:00 [accepted]
PHST- 2004/09/08 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/09/08 05:00 [entrez]
AID - S0165460804000275 [pii]
AID - 10.1016/j.cancergencyto.2004.01.008 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2004 Sep;153(2):115-21. doi: 
      10.1016/j.cancergencyto.2004.01.008.