PMID- 15351620
OWN - NLM
STAT- MEDLINE
DCOM- 20050418
LR  - 20061115
IS  - 1527-9995 (Electronic)
IS  - 0090-4295 (Linking)
VI  - 64
IP  - 3
DP  - 2004 Sep
TI  - Numeric aberrations of HER-2 and chromosome 17 detected by fluorescence in situ 
      hybridization in urine-exfoliated cells from patients with urothelial carcinoma.
PG  - 617-21
AB  - OBJECTIVES: To elucidate the clinical significance of the HER-2 gene alterations 
      in urine-exfoliated cells detected by fluorescence in situ hybridization (FISH) 
      in patients with urothelial transitional cell carcinoma. METHODS: The relative 
      increase of HER-2 (RI-HER2) and gain of chromosome 17 (G-17) in urine-exfoliated 
      cells were examined using DNA probes for HER-2 and the chromosome 17 centromere 
      in 103 patients. In addition, FISH analysis was performed using corresponding 
      paraffin-embedded tissue sections from 45 cases to compare the results obtained 
      using urine-exfoliated cells and those obtained using paraffin-embedded tissue. 
      RESULTS: RI-HER2 and G-17 was found in 23 (22.3%) and 46 (44.6%) of 103 patients, 
      respectively. RI-HER2 was significantly more frequent in tumors with two or more 
      recurrences (40.7% versus 15.8%, P = 0.010) and in those with carcinoma in situ 
      (CIS) (35.4% versus 15.9%, P = 0.029). G-17 was more frequent in high-grade 
      tumors (69.1% versus 16.7%, P = 0.032), invasive tumors (63.6% versus 14.3%, P < 
      0.001), and in patients with CIS (77.1% versus 29.0%, P < 0.001). The positive 
      rate for FISH (presence of RI-HER2 and/or presence of G-17) tended to be more 
      frequent in FISH than in cytology. A comparison of the analyses using 
      urine-exfoliated cells and paraffin-embedded tissue showed identical results in 
      36 (80.0%) of 45 cases. CONCLUSIONS: Numeric alterations of the chromosome 17 
      centromere in urine-exfoliated cells detected by FISH may reflect the malignant 
      potential of urothelial carcinoma. In addition, a relative increase in HER-2 was 
      associated with the number of recurrences and the presence of CIS.
FAU - Inoue, Takamitsu
AU  - Inoue T
AD  - Department of Urology, Akita University School of Medicine, Akita City, Japan.
FAU - Sato, Kazunari
AU  - Sato K
FAU - Tsuchiya, Norihiko
AU  - Tsuchiya N
FAU - Matsuura, Shinobu
AU  - Matsuura S
FAU - Iinuma, Masahiro
AU  - Iinuma M
FAU - Habuchi, Tomonori
AU  - Habuchi T
FAU - Kato, Tetsuro
AU  - Kato T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Urology
JT  - Urology
JID - 0366151
SB  - IM
MH  - Aged
MH  - Aneuploidy
MH  - Carcinoma in Situ/genetics/pathology/urine
MH  - Carcinoma, Transitional Cell/*genetics/pathology/urine
MH  - Centromere/ultrastructure
MH  - *Chromosomes, Human, Pair 17
MH  - Female
MH  - *Gene Amplification
MH  - *Gene Dosage
MH  - *Genes, erbB-2
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local
MH  - Paraffin Embedding
MH  - Urine/*cytology
MH  - Urologic Neoplasms/*genetics/pathology/urine
EDAT- 2004/09/08 05:00
MHDA- 2005/04/19 09:00
CRDT- 2004/09/08 05:00
PHST- 2004/02/09 00:00 [received]
PHST- 2004/05/04 00:00 [accepted]
PHST- 2004/09/08 05:00 [pubmed]
PHST- 2005/04/19 09:00 [medline]
PHST- 2004/09/08 05:00 [entrez]
AID - S0090429504005771 [pii]
AID - 10.1016/j.urology.2004.05.004 [doi]
PST - ppublish
SO  - Urology. 2004 Sep;64(3):617-21. doi: 10.1016/j.urology.2004.05.004.