PMID- 15352546
OWN - NLM
STAT- MEDLINE
DCOM- 20041014
LR  - 20191210
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 68
IP  - 3
DP  - 2004 Jul
TI  - An assessment of sanitation protocols for commercial transport vehicles 
      contaminated with porcine reproductive and respiratory syndrome virus.
PG  - 208-14
AB  - The objective of this study was to develop and test a rapid (< 2 h) sanitation 
      protocol designed for porcine reproductive and respiratory syndrome virus (PRRSV) 
      positive commercial transport vehicles involving cold water washing and 
      disinfection via fumigation using scale models of weaned pig trailers. The study 
      consisted of 2 phases. Following experimental contamination of model trailers 
      with PRRSV MN 30-100 (5 x 10(5)TCID50), phase 1 evaluated the presence or absence 
      of PRRSV RNA by polymerase chain reaction (PCR) on swabs collected from the 
      trailer interiors 0, 60, and 90 min after treatment. Phase 2 consisted of 
      evaluating the infectivity of trailers 90 min posttreatment by monitoring changes 
      in the PRRSV-status of naive sentinel pigs housed for 2 h. Treatments included 
      washing only (treatment 1), washing plus formaldehyde fumigation (treatment 2), 
      washing plus fumigation with glutaraldehyde-quaternary ammonium chloride 
      (treatment 3), and washing plus overnight drying (treatment 4). Porcine 
      reproductive and respiratory syndrome virus RNA was detected in all trailers (20 
      out of 20 replicates) at 60 and 90 min following the application of treatments 1 
      and 2. These trailers also contained infectious PRRSV, as determined by the 
      infection of naive pigs housed in treated trailers and the testing of organic 
      debris collected from the interior of trailers by swine bioassay. At 90 min 
      posttreatment, all trailers treated with glutaraldehyde-quaternary ammonium 
      chloride were PCR-negative, non-infectious to sentinel pigs, and swine bioassay 
      negative. Similar results were observed in trailers allowed to dry for 8 h. Under 
      the conditions of this study, it appears certain disinfectants may possess 
      different levels of efficacy against PRRSV and PRRSV-positive models may be 
      effectively sanitized in the absence of overnight drying.
FAU - Dee, Scott
AU  - Dee S
AD  - Swine Disease Eradication Center, University of Minnesota College of Veterinary 
      Medicine, 1988 Fitch Avenue, St. Paul, Minnesota 55108, USA. deexx004@umn.edu
FAU - Deen, John
AU  - Deen J
FAU - Burns, Danny
AU  - Burns D
FAU - Douthit, George
AU  - Douthit G
FAU - Pijoan, Carlos
AU  - Pijoan C
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - *Disease Reservoirs
MH  - Disinfection/*methods
MH  - Motor Vehicles
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*transmission
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*pathogenicity
MH  - RNA, Viral/analysis
MH  - Swine
PMC - PMC1142141
EDAT- 2004/09/09 05:00
MHDA- 2004/10/16 09:00
PMCR- 2004/10/01
CRDT- 2004/09/09 05:00
PHST- 2004/09/09 05:00 [pubmed]
PHST- 2004/10/16 09:00 [medline]
PHST- 2004/09/09 05:00 [entrez]
PHST- 2004/10/01 00:00 [pmc-release]
AID - pg208 [pii]
PST - ppublish
SO  - Can J Vet Res. 2004 Jul;68(3):208-14.