PMID- 15353131
OWN - NLM
STAT- MEDLINE
DCOM- 20050329
LR  - 20141120
IS  - 1001-0602 (Print)
IS  - 1001-0602 (Linking)
VI  - 14
IP  - 4
DP  - 2004 Aug
TI  - Apoptosis in granulosa cells during follicular atresia: relationship with 
      steroids and insulin-like growth factors.
PG  - 341-6
AB  - It is well known that during mammalian ovarian follicular development, the 
      majority of follicles undergo atresia at various stages of their development. 
      However, the mechanisms controlling this selection process remain unknown. In 
      this study, we investigated apoptosis in granulosa cells during goat follicular 
      atresia by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling 
      (TUNEL). The changes in the levels of steroids, insulin-like growth factors 
      (IGFs) and IGF receptors were studied by radioimmunoassay (RIA) and 
      semi-quantitative reverse transcription-PCR. We found that the percentage of 
      apoptotic granulosa cells in the atretic (A) follicles was significantly higher 
      than that in the slightly atretic (SA) and healthy (H) follicles. The level of 
      estradiol and the ratio of estradiol to progesterone in H follicles were 
      significantly higher than those in A follicles. On the other hand, the level of 
      progesterone was not significantly different among these follicle types. We also 
      found that the level of IGF-I in H follicles was higher than in SA and A 
      follicles, whereas the amount of IGF-II did not vary significantly. The 
      expression of IGF receptor also decreased in A follicles as compared to that in H 
      and SA follicles. These results suggested that estradiol and IGF-I might be 
      involved in controlling apoptosis in granulosa cells during follicular atresia.
FAU - Yu, Yuan Song
AU  - Yu YS
AD  - Laboratory of Animal Reproduction and Embryology College of Animal Sciences and 
      Veterinary Medicine, Shandong Agricultural University, Taian, Shandong 271018, 
      China.
FAU - Sui, Hong Shu
AU  - Sui HS
FAU - Han, Zheng Bin
AU  - Han ZB
FAU - Li, Wei
AU  - Li W
FAU - Luo, Ming Jiu
AU  - Luo MJ
FAU - Tan, Jing He
AU  - Tan JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Res
JT  - Cell research
JID - 9425763
RN  - 0 (Estrogens)
RN  - 0 (Somatomedins)
RN  - 0 (Steroids)
RN  - 3XMK78S47O (Testosterone)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 67763-97-7 (Insulin-Like Growth Factor II)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Estrogens/metabolism
MH  - Female
MH  - Follicular Atresia/*physiology
MH  - Follicular Fluid/metabolism
MH  - Goats
MH  - Granulosa Cells/cytology/*metabolism
MH  - In Situ Nick-End Labeling
MH  - In Vitro Techniques
MH  - Insulin-Like Growth Factor I/metabolism
MH  - Insulin-Like Growth Factor II/metabolism
MH  - Ovarian Follicle/cytology/*metabolism
MH  - Progesterone/metabolism
MH  - Radioimmunoassay
MH  - Receptor, IGF Type 1/metabolism
MH  - Somatomedins/*metabolism
MH  - Steroids/*metabolism
MH  - Testosterone/metabolism
EDAT- 2004/09/09 05:00
MHDA- 2005/03/30 09:00
CRDT- 2004/09/09 05:00
PHST- 2004/09/09 05:00 [pubmed]
PHST- 2005/03/30 09:00 [medline]
PHST- 2004/09/09 05:00 [entrez]
AID - 10.1038/sj.cr.7290234 [doi]
PST - ppublish
SO  - Cell Res. 2004 Aug;14(4):341-6. doi: 10.1038/sj.cr.7290234.