PMID- 15353550
OWN - NLM
STAT- MEDLINE
DCOM- 20041025
LR  - 20181113
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 166
IP  - 6
DP  - 2004 Sep 13
TI  - A novel checkpoint in the Bcl-2-regulated apoptotic pathway revealed by murine 
      cytomegalovirus infection of dendritic cells.
PG  - 827-37
AB  - Infection with murine cytomegalovirus (MCMV) has contributed to understanding 
      many aspects of human infection and, additionally, has provided important insight 
      to understanding complex cellular responses. Dendritic cells (DCs) are a major 
      target for MCMV infection. Here, we analyze the effects of MCMV infection on DC 
      viability, and show that infected DCs become resistant to apoptosis induced by 
      growth factor deprivation. The precise contribution of changes in the expression 
      of Bcl-2 family proteins has been assessed and a new checkpoint in the apoptotic 
      pathway identified. Despite their resistance to apoptosis, MCMV-infected DCs 
      showed Bax to be tightly associated with mitochondria and, together with Bak, 
      forming high molecular weight oligomers, changes normally associated with 
      apoptotic cell death. Exposure of a constitutively occluded Bax NH2-terminal 
      epitope was blocked after infection. These results suggest that MCMV has evolved 
      a novel strategy for inhibiting apoptosis and provide evidence that apoptosis can 
      be regulated after translocation, integration, and oligomerization of Bax at the 
      mitochondrial membrane.
FAU - Andoniou, Christopher E
AU  - Andoniou CE
AD  - Immunology and Virology Program, Centre for Ophthalmology and Visual Science, The 
      University of Western Australia, Western Australia 6009, Australia.
FAU - Andrews, Daniel M
AU  - Andrews DM
FAU - Manzur, Mitali
AU  - Manzur M
FAU - Ricciardi-Castagnoli, Paola
AU  - Ricciardi-Castagnoli P
FAU - Degli-Esposti, Mariapia A
AU  - Degli-Esposti MA
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040907
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Bak1 protein, mouse)
RN  - 0 (Bax protein, mouse)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2 Homologous Antagonist-Killer Protein)
RN  - 0 (bcl-2-Associated X Protein)
SB  - IM
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Cell Line
MH  - Cell Line, Transformed
MH  - Cell Survival
MH  - Cell Transformation, Viral
MH  - Culture Media, Conditioned
MH  - Dendritic Cells/*metabolism
MH  - Gene Expression Regulation
MH  - Herpesviridae Infections/*virology
MH  - Intracellular Membranes/physiology
MH  - Membrane Potentials
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Mitochondria/physiology
MH  - Muromegalovirus/*physiology
MH  - Proto-Oncogene Proteins/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/*metabolism
MH  - bcl-2 Homologous Antagonist-Killer Protein
MH  - bcl-2-Associated X Protein
PMC - PMC2172116
EDAT- 2004/09/09 05:00
MHDA- 2004/10/27 09:00
PMCR- 2005/03/13
CRDT- 2004/09/09 05:00
PHST- 2004/09/09 05:00 [pubmed]
PHST- 2004/10/27 09:00 [medline]
PHST- 2004/09/09 05:00 [entrez]
PHST- 2005/03/13 00:00 [pmc-release]
AID - jcb.200403010 [pii]
AID - 200403010 [pii]
AID - 10.1083/jcb.200403010 [doi]
PST - ppublish
SO  - J Cell Biol. 2004 Sep 13;166(6):827-37. doi: 10.1083/jcb.200403010. Epub 2004 Sep 
      7.