PMID- 15353578 OWN - NLM STAT- MEDLINE DCOM- 20050217 LR - 20220316 IS - 0931-0509 (Print) IS - 0931-0509 (Linking) VI - 19 IP - 11 DP - 2004 Nov TI - Urinary monocyte chemoattractant protein-1 (MCP-1) is a marker of active renal vasculitis. PG - 2761-8 AB - BACKGROUND: Macrophage infiltration and cytokine production are important in the pathogenesis of crescentic glomerulonephritis in anti-neutrophil cytoplasm antibody (ANCA)-associated vasculitis. The aim of this study was to investigate whether urinary levels of chemokines, monocyte chemoattractant protein-1 (MCP-1) and fractalkine, were useful tools for non-invasive assessment of renal vasculitis. METHODS: In a prospective study, concentrations of chemokines were measured in urine and serum samples using specific enzyme-linked immunosorbent assays, and related to the patients' clinical status. Renal expression of MCP-1 was studied by immunohistochemical staining of renal biopsies. RESULTS: Urinary levels of MCP-1 were significantly higher in patients with active (P<0.01) or persistent (P<0.05) renal vasculitis, in comparison with healthy volunteers, control patients, patients with inactive vasculitis and patients with extra-renal disease only. There were no differences in serum concentrations of MCP-1 between these groups. Reduction in urinary MCP-1 levels following treatment preceded the improvement of renal function by a median of 2 weeks. In one patient, rising urinary levels of MCP-1, despite immunosuppressive therapy, was associated with progression to severe renal failure. There were no differences in urinary fractalkine levels between the different groups of patients and controls. Immunohistology of renal biopsies from patients with crescentic glomerulonephritis showed increased staining for MCP-1 in glomerular and interstitial cells. Urinary MCP-1 levels correlated with glomerular, but not tubulointerstitial, macrophage infiltration (P<0.05). CONCLUSIONS: This study shows that measurement of urinary MCP-1, but not fractalkine, is a useful non-invasive technique for the assessment of renal involvement and monitoring the response to therapy in ANCA-associated vasculitis. FAU - Tam, Frederick W K AU - Tam FW AD - Renal Section, Division of Medicine, Faculty of Medicine, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK. f.tam@imperial.ac.uk FAU - Sanders, Jan-Stephan AU - Sanders JS FAU - George, Abraham AU - George A FAU - Hammad, Tarig AU - Hammad T FAU - Miller, Caroline AU - Miller C FAU - Dougan, Tammy AU - Dougan T FAU - Cook, H Terence AU - Cook HT FAU - Kallenberg, Cees G M AU - Kallenberg CG FAU - Gaskin, Gill AU - Gaskin G FAU - Levy, Jeremy B AU - Levy JB FAU - Pusey, Charles D AU - Pusey CD LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20040907 PL - England TA - Nephrol Dial Transplant JT - Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association JID - 8706402 RN - 0 (Biomarkers) RN - 0 (CCL2 protein, human) RN - 0 (CX3CL1 protein, human) RN - 0 (Chemokine CCL2) RN - 0 (Chemokine CX3CL1) RN - 0 (Chemokines, CX3C) RN - 0 (Membrane Proteins) SB - IM MH - Adult MH - Biomarkers/*urine MH - Chemokine CCL2/blood/*urine MH - Chemokine CX3CL1 MH - *Chemokines, CX3C/*urine MH - Female MH - Glomerulonephritis/pathology MH - Humans MH - Kidney Diseases/*urine MH - Male MH - *Membrane Proteins/*urine MH - Middle Aged MH - Prospective Studies MH - Sensitivity and Specificity MH - Vasculitis/*urine EDAT- 2004/09/09 05:00 MHDA- 2005/02/18 09:00 CRDT- 2004/09/09 05:00 PHST- 2004/09/09 05:00 [pubmed] PHST- 2005/02/18 09:00 [medline] PHST- 2004/09/09 05:00 [entrez] AID - gfh487 [pii] AID - 10.1093/ndt/gfh487 [doi] PST - ppublish SO - Nephrol Dial Transplant. 2004 Nov;19(11):2761-8. doi: 10.1093/ndt/gfh487. Epub 2004 Sep 7.