PMID- 15354184
OWN - NLM
STAT- MEDLINE
DCOM- 20050126
LR  - 20161124
IS  - 0893-133X (Print)
IS  - 0893-133X (Linking)
VI  - 30
IP  - 1
DP  - 2005 Jan
TI  - NAAG reduces NMDA receptor current in CA1 hippocampal pyramidal neurons of acute 
      slices and dissociated neurons.
PG  - 7-16
AB  - N-acetylaspartylglutamate (NAAG) is an abundant neuropeptide in the nervous 
      system, yet its functions are not well understood. Pyramidal neurons of the CA1 
      sector of acutely prepared hippocampal slices were recorded using the whole-cell 
      patch-clamp technique. At low concentrations (20 microM), NAAG reduced isolated 
      N-methyl-D-aspartate receptor (NMDAR)-mediated synaptic currents or NMDA-induced 
      currents. The NAAG-induced change in the NMDA concentration/response curve 
      suggested that the antagonism was not competitive. However, the NAAG-induced 
      change in the concentration/response curve for the NMDAR co-agonist, glycine, 
      indicated that glycine can overcome the NAAG antagonism. The antagonism of the 
      NMDAR induced by NAAG was still observed in the presence of LY-341495, a potent 
      and selective mGluR3 antagonist. Moreover, in dissociated pyramidal neurons of 
      the CA1 region, NAAG also reduced the NMDA current and this effect was reversed 
      by glycine. These results suggest that NAAG reduces the NMDA currents in 
      hippocampal CA1 pyramidal neurons.
FAU - Bergeron, Richard
AU  - Bergeron R
AD  - Ottawa Health Research Institute, Ottawa, ON, Canada. rbergeron@ohri.ca
FAU - Coyle, Joseph T
AU  - Coyle JT
FAU - Tsai, Guochan
AU  - Tsai G
FAU - Greene, Robert W
AU  - Greene RW
LA  - eng
GR  - MH 51290/MH/NIMH NIH HHS/United States
GR  - MH606450/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Neuropsychopharmacology
JT  - Neuropsychopharmacology : official publication of the American College of 
      Neuropsychopharmacology
JID - 8904907
RN  - 0 (Dipeptides)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 1W8M12WXYL (isospaglumic acid)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Animals
MH  - Cell Separation
MH  - Dipeptides/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Electric Stimulation
MH  - Electrophysiology
MH  - Glycine/pharmacology
MH  - Hippocampus/cytology/drug effects/*metabolism
MH  - In Vitro Techniques
MH  - Neural Conduction/drug effects
MH  - Neurons/*metabolism
MH  - Patch-Clamp Techniques
MH  - Pyramidal Cells/*metabolism
MH  - Rats
MH  - Rats, Long-Evans
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*antagonists & inhibitors
MH  - Synapses/drug effects
EDAT- 2004/09/09 05:00
MHDA- 2005/01/27 09:00
CRDT- 2004/09/09 05:00
PHST- 2004/09/09 05:00 [pubmed]
PHST- 2005/01/27 09:00 [medline]
PHST- 2004/09/09 05:00 [entrez]
AID - 1300559 [pii]
AID - 10.1038/sj.npp.1300559 [doi]
PST - ppublish
SO  - Neuropsychopharmacology. 2005 Jan;30(1):7-16. doi: 10.1038/sj.npp.1300559.