PMID- 15354743
OWN - NLM
STAT- MEDLINE
DCOM- 20041014
LR  - 20191108
IS  - 1541-2016 (Print)
IS  - 1533-4058 (Linking)
VI  - 12
IP  - 2
DP  - 2004 Jun
TI  - Ewing sarcoma/peripheral primitive neuroectodermal tumor: adult abdominal tumors 
      with an Ewing sarcoma gene rearrangement demonstrated by fluorescence in situ 
      hybridization in paraffin sections.
PG  - 160-5
AB  - The differential diagnosis of small round cell tumors is exhaustive and requires 
      ancillary studies. Relatively recently, fluorescence in situ hybridization (FISH) 
      using probes for specific gene rearrangements has gained wide acceptance. This 
      technique is particularly useful in the differential diagnosis of Ewing 
      sarcoma/primitive neuroectodermal tumor (ES/PNET) and desmoplastic small 
      round-cell tumor (DSRCT). In ES/PNET, the EWS gene is juxtaposed to the FLI-1 
      gene in 85% of cases and to the ERG gene in another 7% of cases; the EWS gene is 
      juxtaposed to the WTI gene in DSRCT. Documentation of the EWS gene rearrangements 
      in EWS/PNET has previously been demonstrated in frozen tissue. We report 2 
      unusual cases of EWS/PNET diagnosed in abdominal tumors in adults. Although the 
      immunohistochemical results supported a diagnosis of ES/PNET, 1 case 
      morphologically resembled DSRCT. The diagnosis in these 2 cases was confirmed by 
      the FISH demonstration of EWS/FLI-1 gene fusion in paraffin-embedded tissue. 
      Thus, the usefulness of FISH demonstration of an EWS gene rearrangement with 
      these specific probes in such unusual cases is supported and is demonstrated in 
      paraffin-embedded tissue.
FAU - Gardner, Laura J
AU  - Gardner LJ
AD  - Division of Hematopathology, Department of Pathology, St. Louis University Health 
      Sciences Center, St. Louis, Missouri, USA.
FAU - Ayala, Alberto G
AU  - Ayala AG
FAU - Monforte, Hector L
AU  - Monforte HL
FAU - Dunphy, Cherie H
AU  - Dunphy CH
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Appl Immunohistochem Mol Morphol
JT  - Applied immunohistochemistry & molecular morphology : AIMM
JID - 100888796
RN  - 0 (EWS-FLI fusion protein)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Protein c-fli-1)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Abdominal Neoplasms/diagnosis/genetics
MH  - Carcinoma, Small Cell/pathology
MH  - Diagnosis, Differential
MH  - Female
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neuroectodermal Tumors, Primitive, Peripheral/*diagnosis/genetics
MH  - Oncogene Proteins, Fusion/genetics
MH  - Paraffin Embedding
MH  - Proto-Oncogene Protein c-fli-1
MH  - RNA-Binding Protein EWS/*genetics
MH  - Sarcoma, Ewing/*diagnosis/genetics
MH  - Transcription Factors/genetics
EDAT- 2004/09/10 05:00
MHDA- 2004/10/16 09:00
CRDT- 2004/09/10 05:00
PHST- 2004/09/10 05:00 [pubmed]
PHST- 2004/10/16 09:00 [medline]
PHST- 2004/09/10 05:00 [entrez]
AID - 10.1097/00129039-200406000-00011 [doi]
PST - ppublish
SO  - Appl Immunohistochem Mol Morphol. 2004 Jun;12(2):160-5. doi: 
      10.1097/00129039-200406000-00011.