PMID- 15355471
OWN - NLM
STAT- MEDLINE
DCOM- 20050412
LR  - 20191210
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 59
IP  - 10
DP  - 2004 Oct
TI  - The CD63 basophil activation test in Hymenoptera venom allergy: a prospective 
      study.
PG  - 1110-7
AB  - BACKGROUND: The basophil activation test (BAT), which relies on flow cytometric 
      quantitation of the allergen-induced up-regulation of the granule-associated 
      marker CD63 in peripheral blood basophils, has been suggested to be a useful 
      approach in detecting responsiveness to allergens. The purpose of this study was 
      to establish the usefulness of the BAT with regard to the clinical history and 
      current diagnostic tools in Hymenoptera venom allergy using a prospective study 
      design. METHODS: Fifty-seven consecutive patients allergic to Hymenoptera venom 
      as defined by a systemic reaction after an insect sting, and 30 age- and 
      sex-matched control subjects with a negative history were included. The degree 
      and nature of sensitization was confirmed by skin testing, specific 
      immunoglobulin E (IgE), serum tryptase levels and BAT. In the nonallergic control 
      group only analysis of specific IgE and BAT were performed. Correlation of BAT, 
      skin test and specific IgE, respectively, with the clinical history in the 
      allergic group was termed as sensitivity and in the control group as specificity. 
      RESULTS: Twenty one of 23 (91.3%) bee venom allergic patients and 29 of 34 
      (85.3%) patients allergic to wasp and hornet venom tested positive in BAT. The 
      overall sensitivity of BAT, specific IgE and skin tests were 87.7, 91.2 and 
      93.0%, respectively. The overall specificities were 86.7% for BAT and 66.7% for 
      specific IgE. No correlation between the severity of clinical symptoms and the 
      magnitude of basophil activation was observed. CONCLUSION: The BAT seems to be an 
      appropriate method to identify patients allergic to bee or wasp venom with a 
      comparable sensitivity to standard diagnostic regimens. The higher specificity of 
      BAT as compared with specific IgE makes this test a useful tool in the diagnosis 
      of Hymenoptera venom allergy.
FAU - Sturm, G J
AU  - Sturm GJ
AD  - Department of Experimental and Clinical Pharmacology, University of Graz, Graz, 
      Austria.
FAU - Bohm, E
AU  - Bohm E
FAU - Trummer, M
AU  - Trummer M
FAU - Weiglhofer, I
AU  - Weiglhofer I
FAU - Heinemann, A
AU  - Heinemann A
FAU - Aberer, W
AU  - Aberer W
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Antigens, CD)
RN  - 0 (Arthropod Venoms)
RN  - 0 (CD63 protein, human)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Tetraspanin 30)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.59 (Tryptases)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*immunology
MH  - Arthropod Venoms/*immunology
MH  - Basophils/*immunology
MH  - Humans
MH  - Hymenoptera/*immunology
MH  - Hypersensitivity/*immunology
MH  - Immunoglobulin E/immunology
MH  - Insect Bites and Stings/*immunology
MH  - Platelet Membrane Glycoproteins/*immunology
MH  - Prospective Studies
MH  - Serine Endopeptidases/immunology
MH  - Skin Tests
MH  - Tetraspanin 30
MH  - Tryptases
EDAT- 2004/09/10 05:00
MHDA- 2005/04/13 09:00
CRDT- 2004/09/10 05:00
PHST- 2004/09/10 05:00 [pubmed]
PHST- 2005/04/13 09:00 [medline]
PHST- 2004/09/10 05:00 [entrez]
AID - ALL400 [pii]
AID - 10.1111/j.1398-9995.2004.00400.x [doi]
PST - ppublish
SO  - Allergy. 2004 Oct;59(10):1110-7. doi: 10.1111/j.1398-9995.2004.00400.x.