PMID- 15356021
OWN - NLM
STAT- MEDLINE
DCOM- 20041007
LR  - 20120604
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 89
IP  - 9
DP  - 2004 Sep
TI  - Heterogeneity in the distribution of RET/PTC rearrangements within individual 
      post-Chernobyl papillary thyroid carcinomas.
PG  - 4272-9
AB  - The nuclear disaster that occurred in Chernobyl in 1986 offered the unique 
      opportunity to study the molecular genetics of one human tumor type, papillary 
      carcinoma of the thyroid gland, associated with a specific etiology. We have 
      analyzed RET rearrangements in post-Chernobyl papillary thyroid carcinomas (n = 
      29), follicular thyroid adenomas (n = 2), and follicular thyroid carcinoma (n = 
      1) by interphase fluorescence in situ hybridization (FISH) analysis on 
      paraffin-embedded tissue sections. Paraffin sections were microdissected before 
      use to ensure that only tumor was present. Cell nuclei were scored for the 
      presence of a split FISH signal (separated red and green signal) in addition to 
      an overlapping signal. Only cells with either two overlapping signals or one 
      split and one overlapping signal were counted to ensure that only complete cell 
      nuclei had been scored. In total, 23 of 32 cases (72%) showed RET rearrangements 
      diagnosed by FISH interphase analysis. In all cases, the tumors were composed of 
      a mixture of cells with and without ret rearrangement on FISH. In some cases, 
      this distribution was clearly nonrandom because clustering of rearranged cells 
      was detected within the same tumor nodule. Accordingly, only 31% of the cases 
      positive for rearrangement on FISH also scored positive using RT-PCR. These 
      findings suggest that because RET/PTC rearrangements are not present in a 
      majority of tumor cells, either a fraction of post-Chernobyl papillary thyroid 
      tumors are of multiclonal origin, or ret rearrangement is a later, subclonal 
      event.
FAU - Unger, Kristian
AU  - Unger K
AD  - GSF-Forschungszentrum fur Umwelt und Gesundheit GmbH, Institute of Molecular 
      Radiobiology, Ingolstadter Landstrasse 1, D-85764 Neuherberg, Germany.
FAU - Zitzelsberger, Horst
AU  - Zitzelsberger H
FAU - Salvatore, Giuliana
AU  - Salvatore G
FAU - Santoro, Massimo
AU  - Santoro M
FAU - Bogdanova, Tatjana
AU  - Bogdanova T
FAU - Braselmann, Herbert
AU  - Braselmann H
FAU - Kastner, Peter
AU  - Kastner P
FAU - Zurnadzhy, Lyudmilla
AU  - Zurnadzhy L
FAU - Tronko, Nikolay
AU  - Tronko N
FAU - Hutzler, Peter
AU  - Hutzler P
FAU - Thomas, Gerry
AU  - Thomas G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Oncogene Proteins)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-ret)
RN  - EC 2.7.10.1 (RET protein, human)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
CIN - J Clin Endocrinol Metab. 2004 Sep;89(9):4264-6. PMID: 15356019
MH  - Adolescent
MH  - Carcinoma, Papillary/*genetics
MH  - Child
MH  - Female
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neoplasms, Radiation-Induced/*genetics
MH  - Oncogene Proteins/*genetics
MH  - *Power Plants
MH  - Proto-Oncogene Proteins c-ret
MH  - *Radioactive Hazard Release
MH  - Receptor Protein-Tyrosine Kinases/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Thyroid Neoplasms/*genetics
MH  - Ukraine
EDAT- 2004/09/10 05:00
MHDA- 2004/10/08 09:00
CRDT- 2004/09/10 05:00
PHST- 2004/09/10 05:00 [pubmed]
PHST- 2004/10/08 09:00 [medline]
PHST- 2004/09/10 05:00 [entrez]
AID - 89/9/4272 [pii]
AID - 10.1210/jc.2003-031870 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2004 Sep;89(9):4272-9. doi: 10.1210/jc.2003-031870.