PMID- 15356554
OWN - NLM
STAT- MEDLINE
DCOM- 20041020
LR  - 20131121
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 114
IP  - 3
DP  - 2004 Sep
TI  - Effects of antiasthmatic agents on the functions of peripheral blood 
      monocyte-derived dendritic cells from atopic patients.
PG  - 538-44
AB  - BACKGROUND: Dendritic cells (DCs), the antigen-presenting cells in the airway, 
      play a critical role in asthma. Nevertheless, there is little information on the 
      effects of antiasthmatic agents on DCs. OBJECTIVES: The purpose of the present 
      study was to determine the effects of representative antiasthmatic agents, 
      including cysteinyl leukotriene (cysLT) 1 receptor antagonists, corticosteroid, 
      and tacrolimus, on DCs in inducing allergy. METHODS: Human peripheral blood 
      monocyte-derived DCs (MoDCs) generated from atopic and healthy subjects were 
      pulsed with Dermatophagoides farinae allergen in the presence of medium alone, 
      pranlukast, montelukast, dexamethasone, or tacrolimus. The mRNA expressions of 
      cysLT receptor, cysLTs producing enzymes, and various surface markers on MoDCs, 
      as well as the concentrations of cysLTs, IL-10, and IL-12 in cultured 
      supernatants, were determined. MoDCs were also cocultured in vitro with 
      autologous CD4(+) T cells, and IL-5 and IFN-gamma production was measured. 
      RESULTS: MoDCs of atopic patients expressed mRNAs of cysLT1 receptor and 
      cysLT-producing enzymes, and allergen pulsing significantly increased cysLT 
      production. MoDCs of atopic patients showed a T(H)2-favoring phenotype and 
      induced T(H)2-skewed cytokine production from autologous CD4(+) T cells. 
      Dexamethasone and tacrolimus inhibited allergen-pulsed MoDC-induced cytokine 
      production by autologous CD4(+) T cells without and with IL-10 inhibition, 
      respectively. CysLT1 receptor antagonists had no effect on MoDC functions. 
      CONCLUSION: Our results indicate that MoDCs of atopic patients induce a T(H)2 
      reaction. Corticosteroid and tacrolimus, but not cysLT1 receptor antagonists, 
      inhibit T(H)2 reactions, and this effect is probably mediated through different 
      pathways.
FAU - Saeki, Sachiko
AU  - Saeki S
AD  - Second Department of Internal Medicine, Nagasaki University School of Medicine, 
      Nagasaki 852-8501, Japan.
FAU - Matsuse, Hiroto
AU  - Matsuse H
FAU - Kondo, Yuki
AU  - Kondo Y
FAU - Machida, Ikuko
AU  - Machida I
FAU - Kawano, Tetsuya
AU  - Kawano T
FAU - Tomari, Shinya
AU  - Tomari S
FAU - Obase, Yasushi
AU  - Obase Y
FAU - Fukushima, Chizu
AU  - Fukushima C
FAU - Kohno, Shigeru
AU  - Kohno S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (Anti-Asthmatic Agents)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Cytokines)
RN  - 0 (Leukotriene Antagonists)
RN  - 0 (Leukotrienes)
RN  - 0 (cysteinyl-leukotriene)
RN  - K848JZ4886 (Cysteine)
RN  - WM0HAQ4WNM (Tacrolimus)
SB  - IM
MH  - Adrenal Cortex Hormones/administration & dosage/pharmacology
MH  - Adult
MH  - Anti-Asthmatic Agents/*pharmacology/therapeutic use
MH  - Antigens, Dermatophagoides/immunology
MH  - CD4-Positive T-Lymphocytes/immunology/metabolism
MH  - Cell Differentiation
MH  - Cysteine/metabolism
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*drug effects
MH  - Female
MH  - Humans
MH  - Hypersensitivity, Immediate/*drug therapy/immunology
MH  - Leukotriene Antagonists/administration & dosage/pharmacology
MH  - Leukotrienes/metabolism
MH  - Male
MH  - Monocytes/*cytology
MH  - Tacrolimus/administration & dosage/pharmacology
EDAT- 2004/09/10 05:00
MHDA- 2004/10/22 09:00
CRDT- 2004/09/10 05:00
PHST- 2004/09/10 05:00 [pubmed]
PHST- 2004/10/22 09:00 [medline]
PHST- 2004/09/10 05:00 [entrez]
AID - S0091674904015672 [pii]
AID - 10.1016/j.jaci.2004.05.053 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2004 Sep;114(3):538-44. doi: 10.1016/j.jaci.2004.05.053.