PMID- 15357284
OWN - NLM
STAT- MEDLINE
DCOM- 20041021
LR  - 20131121
IS  - 1225-8873 (Print)
IS  - 1225-8873 (Linking)
VI  - 42
IP  - 1
DP  - 2004 Mar
TI  - Denaturing gradient gel electrophoresis analysis of bacterial populations in 
      5-stage biological nutrient removal process with step feed system for wastewater 
      treatment.
PG  - 1-8
AB  - Changes in the bacterial populations of a 5-stage biological nutrient removal 
      (BNR) process, with a step feed system for wastewater treatment, were monitored 
      by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal 
      DNA fragments. DGGE analysis indicated seasonal community changes were observed, 
      however, community profiles of the total bacteria of each reactor showed only 
      minor differences in the samples obtained from the same season. The number of 
      major bands was higher in the summer samples, and decreased during the winter 
      period, indicating that the microbial community structure became simpler at low 
      temperatures. Since the nitrogen and phosphate removal efficiencies were highly 
      maintained throughout the winter operation period, the bacteria which still 
      remaining in the winter sample can be considered important, playing a key role in 
      the present 5-stage BNR sludge. The prominent DGGE bands were excised, and 
      sequenced to gain insight into the identities of the predominant bacterial 
      populations present, and most were found to not be closely related to previously 
      characterized bacteria. These data suggest the importance of culture-independent 
      methods for the quality control of wastewater treatment.
FAU - Lee, Soo-Youn
AU  - Lee SY
AD  - School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, 
      Korea.
FAU - Kim, Hyeon-Guk
AU  - Kim HG
FAU - Park, Jong Bok
AU  - Park JB
FAU - Park, Yong Keun
AU  - Park YK
LA  - eng
SI  - GENBANK/AY362830
SI  - GENBANK/AY362840
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Microbiol
JT  - Journal of microbiology (Seoul, Korea)
JID - 9703165
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Phosphates)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Bacteria/genetics/*growth & development
MH  - DNA, Bacterial/analysis/isolation & purification
MH  - DNA, Ribosomal/*analysis/chemistry
MH  - *Electrophoresis, Polyacrylamide Gel
MH  - Genes, rRNA
MH  - Molecular Sequence Data
MH  - Nitrogen/metabolism
MH  - Nucleic Acid Denaturation
MH  - Phosphates/metabolism
MH  - Phylogeny
MH  - Population Dynamics
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seasons
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - Waste Disposal, Fluid
MH  - *Water Purification
EDAT- 2004/09/11 05:00
MHDA- 2004/10/22 09:00
CRDT- 2004/09/11 05:00
PHST- 2004/09/11 05:00 [pubmed]
PHST- 2004/10/22 09:00 [medline]
PHST- 2004/09/11 05:00 [entrez]
AID - 2009 [pii]
PST - ppublish
SO  - J Microbiol. 2004 Mar;42(1):1-8.