PMID- 15357730
OWN - NLM
STAT- MEDLINE
DCOM- 20050106
LR  - 20040910
IS  - 1364-5072 (Print)
IS  - 1364-5072 (Linking)
VI  - 97
IP  - 4
DP  - 2004
TI  - Morphological and phenotypical characterization of Bacillus sporothermodurans.
PG  - 802-9
AB  - AIMS: Enumeration of resistant bacteria in ultra-high temperature (UHT) treated 
      milk; morphological characterization and phenotyping of resistant strains by 
      traditional and nontraditional methods and their identification by molecular 
      biology. METHODS AND RESULTS: Modified standard plate count agar (PCA) and 
      modified brain-heart infusion (BHI) agar were used for colony counts. 
      Physiological culture traits were determined as suggested by Bergey's Manual of 
      Systematic Bacteriology or in modified J-broth or in modified BHI agar. Scanning 
      electron microscope (SEM) was used for microscopic examination. Strain 
      identification was carried out by polymerase chain reaction (PCR). A total of 125 
      (62.81% of 199) samples were positive and the bacterial load was higher than 
      10(5) CFU ml(-1) in 46 samples (28.80% of 125). The 16S rRNA sequence of 
      bacterial cultures obtained from UHT-treated milk was similar to that of Bacillus 
      sporothermodurans M215 type strain((T)) and different biotypes were found by 
      analysis of colony appearance, cell morphology and physiological traits. 
      CONCLUSIONS: Bacillus sporothermodurans was the predominant sporigenous 
      micro-organisms in UHT milk. SIGNIFICANCE AND IMPACT OF THE STUDY: BHI agar is 
      more suitable than PCA for quality control of milk after UHT treatment. Modified 
      J-broth medium is useful to determine selected physiological traits of B. 
      sporothermodurans. The strains characterized and identified as B. 
      sporothermodurans were significantly different compared with the type strain.
FAU - Montanari, G
AU  - Montanari G
AD  - DIPROVAL, Universita di Bologna, Bologna, Italy. grazia@stpa.unibo.it
FAU - Borsari, A
AU  - Borsari A
FAU - Chiavari, C
AU  - Chiavari C
FAU - Ferri, G
AU  - Ferri G
FAU - Zambonelli, C
AU  - Zambonelli C
FAU - Grazia, L
AU  - Grazia L
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Culture Media)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacillus/*genetics/metabolism/ultrastructure
MH  - Colony Count, Microbial/methods
MH  - Culture Media
MH  - *Food Microbiology
MH  - Microscopy, Electron, Scanning/methods
MH  - Milk/*microbiology
MH  - Phenotype
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sterilization
MH  - Temperature
EDAT- 2004/09/11 05:00
MHDA- 2005/01/07 09:00
CRDT- 2004/09/11 05:00
PHST- 2004/09/11 05:00 [pubmed]
PHST- 2005/01/07 09:00 [medline]
PHST- 2004/09/11 05:00 [entrez]
AID - JAM2371 [pii]
AID - 10.1111/j.1365-2672.2004.02371.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2004;97(4):802-9. doi: 10.1111/j.1365-2672.2004.02371.x.