PMID- 15358210
OWN - NLM
STAT- MEDLINE
DCOM- 20041110
LR  - 20071114
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 321
IP  - 1
DP  - 2004 Aug 13
TI  - A novel phosphoprotein is induced during bone marrow commitment to dendritic 
      cells.
PG  - 26-30
AB  - Dendritic cells (DCs) play an important role in vertebrate immunity, but little 
      is known of the molecular events associated with their development from bone 
      marrow (BM). This report describes induction of a signature protein marking BM 
      commitment to DCs. Using a standard procedure, DCs were generated from BM by 
      cultivation in vitro. Appropriate phenotypic monitoring was done primarily by 
      immunofluorescence, and polyclonal antibody reagents were developed against 
      immature DC lysates. Using one specific antibody reagent, we identified, 
      purified, and sequenced a unique cytosolic phosphoprotein DP58 that occurs within 
      30 min during BM commitment to DCs. Its sequence matches with a computationally 
      predicted Riken cDNA (GenBank Accession No. XP_138799), and a specific anti-DP58 
      peptide antibody was developed for further characterization. The study suggests 
      that DP58 induction signals distinct pathway(s) leading to early DC progenitors 
      that may be generated and propagated for a short period in vitro.
FAU - Al-Shaibi, Nisreen
AU  - Al-Shaibi N
AD  - Department of Life Sciences, Indiana State University, Terre Haute, IN 47809, 
      USA.
FAU - Ghosh, Swapan K
AU  - Ghosh SK
LA  - eng
GR  - R15CA70914/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, Surface)
RN  - 0 (Phosphoproteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Surface/analysis
MH  - Bone Marrow Cells/*cytology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*immunology
MH  - Immunophenotyping
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Phosphoproteins/*biosynthesis/chemistry/isolation & purification
MH  - Rabbits
MH  - Stem Cells/*cytology
EDAT- 2004/09/11 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/09/11 05:00
PHST- 2004/06/17 00:00 [received]
PHST- 2004/09/11 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/09/11 05:00 [entrez]
AID - S0006-291X(04)01387-7 [pii]
AID - 10.1016/j.bbrc.2004.06.106 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2004 Aug 13;321(1):26-30. doi: 
      10.1016/j.bbrc.2004.06.106.