PMID- 15358646
OWN - NLM
STAT- MEDLINE
DCOM- 20050503
LR  - 20181113
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 11
IP  - 5
DP  - 2004 Sep
TI  - Analysis of amino acid sequence variations and immunoglobulin E-binding epitopes 
      of German cockroach tropomyosin.
PG  - 874-8
AB  - The allergenicities of tropomyosins from different organisms have been reported 
      to vary. The cDNA encoding German cockroach tropomyosin (Bla g 7) was isolated, 
      expressed, and characterized previously. In the present study, the amino acid 
      sequence variations in German cockroach tropomyosin were analyzed in order to 
      investigate its influence on allergenicity. We also undertook the identification 
      of immunodominant peptides containing immunoglobulin E (IgE) epitopes which may 
      facilitate the development of diagnostic and immunotherapeutic strategies based 
      on the recombinant proteins. Two-dimensional gel electrophoresis and immunoblot 
      analysis with mouse anti-recombinant German cockroach tropomyosin serum was 
      performed to investigate the isoforms at the protein level. Reverse transcriptase 
      PCR (RT-PCR) was applied to examine the sequence diversity. Eleven different 
      variants of the deduced amino acid sequences were identified by RT-PCR. German 
      cockroach tropomyosin has only minor sequence variations that did not seem to 
      affect its allergenicity significantly. These results support the molecular basis 
      underlying the cross-reactivities of arthropod tropomyosins. Recombinant 
      fragments were also generated by PCR, and IgE-binding epitopes were assessed by 
      enzyme-linked immunosorbent assay. Sera from seven patients revealed 
      heterogeneous IgE-binding responses. This study demonstrates multiple IgE-binding 
      epitope regions in a single molecule, suggesting that full-length tropomyosin 
      should be used for the development of diagnostic and therapeutic reagents.
FAU - Jeong, Kyoung Yong
AU  - Jeong KY
AD  - Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 
      Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
FAU - Lee, Jongweon
AU  - Lee J
FAU - Lee, In-Yong
AU  - Lee IY
FAU - Ree, Han-Il
AU  - Ree HI
FAU - Hong, Chein-Soo
AU  - Hong CS
FAU - Yong, Tai-Soon
AU  - Yong TS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (DNA, Complementary)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Protein Isoforms)
RN  - 0 (Tropomyosin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen-Antibody Reactions
MH  - Cockroaches/*immunology
MH  - Cross Reactions/genetics/immunology
MH  - DNA, Complementary
MH  - Immunodominant Epitopes/*genetics
MH  - Immunoglobulin E/*immunology
MH  - Protein Isoforms
MH  - *Sequence Analysis, Protein
MH  - Tropomyosin/genetics/*immunology
PMC - PMC515276
EDAT- 2004/09/11 05:00
MHDA- 2005/05/04 09:00
PMCR- 2004/09/01
CRDT- 2004/09/11 05:00
PHST- 2004/09/11 05:00 [pubmed]
PHST- 2005/05/04 09:00 [medline]
PHST- 2004/09/11 05:00 [entrez]
PHST- 2004/09/01 00:00 [pmc-release]
AID - 11/5/874 [pii]
AID - 0095-04 [pii]
AID - 10.1128/CDLI.11.5.874-878.2004 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2004 Sep;11(5):874-8. doi: 
      10.1128/CDLI.11.5.874-878.2004.