PMID- 15358648
OWN - NLM
STAT- MEDLINE
DCOM- 20050503
LR  - 20181113
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 11
IP  - 5
DP  - 2004 Sep
TI  - Different methods of identifying new antigenic epitopes of human papillomavirus 
      type 16 E6 and E7 proteins.
PG  - 889-96
AB  - Human papillomavirus (HPV) infection is the most common cause of sexually 
      transmitted viral infection and is the main cause of cervical cancer. 
      Identification of HPV T-cell epitopes would be instrumental not only in our 
      understanding of the protective immune response but also in the development of 
      vaccines and immunotherapies. In contrast to viruses which cause systemic 
      infection, identification of HPV epitopes is technically challenging because HPV 
      causes a localized mucosal infection and the frequency of pathogen-specific T 
      lymphocytes in peripheral blood is expected to be low. Here we describe three new 
      antigenic epitopes (E7 7-15 [TLHEYMLDL], E6 52-61 [FAFRDLCIVY], and E7 79-87 
      [LEDLLMGTL]) of HPV 16 E6 and E7 proteins which have oncogenic activities. E7 
      7-15 was identified among peptides previously shown to bind to human leukocyte 
      antigen (HLA)-A2.1 molecule, but it was found likely to be restricted by the 
      HLA-B48 molecule. E6 52-61 (likely to be restricted by HLA-B57) and E7 79-87 
      (likely to be restricted by HLA-B60) were detected, based on the magnitude of the 
      T-cell immune responses, in another individual. In particular, T-cell clones 
      specific for the E6 52-61 epitope were isolated effectively by magnetically 
      selecting them based on gamma interferon secretion. This is an efficient method 
      of identifying new epitopes of antigens for which the number of specific T 
      lymphocytes in the circulation is expected to be small, and it should be widely 
      applicable in identifying new T-cell epitopes.
FAU - Nakagawa, Mayumi
AU  - Nakagawa M
AD  - Department of Laboratory Medicine, School of Medicine, University of California 
      at San Francisco, San Francisco, California, USA. nakagawamayumi@uams.edu
FAU - Kim, Kevin H
AU  - Kim KH
FAU - Moscicki, Anna-Barbara
AU  - Moscicki AB
LA  - eng
GR  - R01 CA051323/CA/NCI NIH HHS/United States
GR  - K07 CA75974/CA/NCI NIH HHS/United States
GR  - CA51323/CA/NCI NIH HHS/United States
GR  - M01 RR01271/RR/NCRR NIH HHS/United States
GR  - M01 RR001271/RR/NCRR NIH HHS/United States
GR  - R37 CA051323/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Antigens, Viral)
RN  - 0 (E6 protein, Human papillomavirus type 16)
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Papillomavirus E7 Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (oncogene protein E7, Human papillomavirus type 16)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Viral/analysis/*isolation & purification
MH  - Clone Cells
MH  - Epitope Mapping
MH  - Epitopes/analysis/*isolation & purification
MH  - HLA Antigens
MH  - Humans
MH  - Immunomagnetic Separation
MH  - Oncogene Proteins, Viral/*immunology
MH  - Papillomavirus E7 Proteins
MH  - Repressor Proteins/*immunology
MH  - T-Lymphocytes/cytology/immunology
PMC - PMC515262
EDAT- 2004/09/11 05:00
MHDA- 2005/05/04 09:00
PMCR- 2004/09/01
CRDT- 2004/09/11 05:00
PHST- 2004/09/11 05:00 [pubmed]
PHST- 2005/05/04 09:00 [medline]
PHST- 2004/09/11 05:00 [entrez]
PHST- 2004/09/01 00:00 [pmc-release]
AID - 11/5/889 [pii]
AID - 0044-04 [pii]
AID - 10.1128/CDLI.11.5.889-896.2004 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2004 Sep;11(5):889-96. doi: 
      10.1128/CDLI.11.5.889-896.2004.