PMID- 15358815
OWN - NLM
STAT- MEDLINE
DCOM- 20041116
LR  - 20200318
IS  - 0022-2615 (Print)
IS  - 0022-2615 (Linking)
VI  - 53
IP  - Pt 10
DP  - 2004 Oct
TI  - Sorting a Staphylococcus aureus phage display library against ex vivo 
      biomaterial.
PG  - 945-951
LID - 10.1099/jmm.0.45638-0 [doi]
AB  - A phage display library made from Staphylococcus aureus DNA was sorted against a 
      central venous catheter (CVC) that had been removed from a patient 2 days after 
      insertion. After the first panning, approximately 50% of the clones encoded 
      proteins known to interact with mammalian proteins. After the second and third 
      pannings, fibrinogen-binding and beta2-glycoprotein I (beta2-GPI)-binding phage 
      particles were clearly dominating. Proteins adsorbed to different CVCs were 
      investigated using specific antibodies. Among the proteins probed for, fibrinogen 
      was most abundant, but, interestingly, beta2-GPI was also detected on all tested 
      CVCs.
FAU - Bjerketorp, Joakim
AU  - Bjerketorp J
AD  - Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 
      07 Uppsala, Sweden.
FAU - Rosander, Anna
AU  - Rosander A
AD  - Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 
      07 Uppsala, Sweden.
FAU - Nilsson, Martin
AU  - Nilsson M
AD  - Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 
      07 Uppsala, Sweden.
FAU - Jacobsson, Karin
AU  - Jacobsson K
AD  - Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 
      07 Uppsala, Sweden.
FAU - Frykberg, Lars
AU  - Frykberg L
AD  - Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 
      07 Uppsala, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Bacterial Proteins)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Carrier Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Peptide Library)
RN  - 0 (Sbi protein, Staphylococcus aureus)
RN  - 0 (beta 2-Glycoprotein I)
RN  - 9001-32-5 (Fibrinogen)
SB  - IM
MH  - *Bacterial Adhesion
MH  - Bacterial Proteins/metabolism
MH  - *Biocompatible Materials
MH  - Carrier Proteins/metabolism
MH  - *Catheterization, Central Venous
MH  - Fibrinogen/metabolism
MH  - Glycoproteins/metabolism
MH  - Peptide Library
MH  - Protein Binding
MH  - Staphylococcus aureus/*genetics/physiology
MH  - beta 2-Glycoprotein I
EDAT- 2004/09/11 05:00
MHDA- 2004/11/17 09:00
CRDT- 2004/09/11 05:00
PHST- 2004/09/11 05:00 [pubmed]
PHST- 2004/11/17 09:00 [medline]
PHST- 2004/09/11 05:00 [entrez]
AID - 10.1099/jmm.0.45638-0 [doi]
PST - ppublish
SO  - J Med Microbiol. 2004 Oct;53(Pt 10):945-951. doi: 10.1099/jmm.0.45638-0.