PMID- 15361122
OWN - NLM
STAT- MEDLINE
DCOM- 20050224
LR  - 20101118
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 64
IP  - 4
DP  - 2004 Oct
TI  - High level of chromosome 15 aneuploidy in head and neck squamous cell carcinoma 
      lesions identified by FISH analysis: limited value of beta2-microglobulin LOH 
      analysis.
PG  - 452-61
AB  - In cancer research, loss of heterozygosity (LOH), defined by microsatellite 
      markers, is frequently used in the identification of gene loss. Especially, 
      genomic alterations in the human leukocyte antigen (HLA) genes and the 
      beta2-microglobulin (beta2m) gene on chromosome 15 are of interest regarding 
      their function in the immune system. Because LOH analysis detects any allelic 
      imbalance and not just allelic loss, we evaluated the LOH analysis in 11 head and 
      neck squamous cell carcinoma (HNSCC) lesions using fluorescence in situ 
      hybridization (FISH). The 11 tumors were selected out of 53 HNSCC lesions based 
      upon beta2m LOH analysis and beta2m expression. Centromere 1 and 15 FISH were 
      developed to determine the chromosome 15 copy number. Sequence-based mutation 
      analysis of beta2m was conducted on tumors without beta2m expression; no 
      mutations in the coding sequences were found. For five HNSCC lesions with LOH and 
      beta2m expression, centromere 15 FISH indicated gain rather than loss. In the 
      majority of the 11 HNSCC lesions, FISH showed centromere 1 and 15 heterogeneity 
      throughout the tumor. Moreover, FISH indicated a more complex chromosome 1 and 15 
      distribution than could be concluded from microsatellite LOH analysis. Our 
      results show that microsatellite LOH analysis does not represent the beta2m gene 
      copy number and support the results obtained from comparative genomic 
      hybridization (CGH) studies. Conclusions on genomic alterations in tumors cannot 
      be based on LOH data only but depend on the results of immunohistochemical 
      staining, FISH, and CGH.
FAU - Koene, G J P A
AU  - Koene GJ
AD  - Department of Pathology, University Medical Center, Utrecht, The Netherlands.
FAU - Arts-Hilkes, Y H A
AU  - Arts-Hilkes YH
FAU - van der Ven, K J W
AU  - van der Ven KJ
FAU - Rozemuller, E H
AU  - Rozemuller EH
FAU - Slootweg, P J
AU  - Slootweg PJ
FAU - de Weger, R A
AU  - de Weger RA
FAU - Tilanus, M G J
AU  - Tilanus MG
LA  - eng
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (beta 2-Microglobulin)
SB  - IM
MH  - Allelic Imbalance/genetics
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 15/*genetics
MH  - Genetic Linkage
MH  - Head and Neck Neoplasms/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Loss of Heterozygosity/*genetics
MH  - beta 2-Microglobulin/*genetics
EDAT- 2004/09/14 05:00
MHDA- 2005/02/25 09:00
CRDT- 2004/09/14 05:00
PHST- 2004/09/14 05:00 [pubmed]
PHST- 2005/02/25 09:00 [medline]
PHST- 2004/09/14 05:00 [entrez]
AID - TAN291 [pii]
AID - 10.1111/j.1399-0039.2004.00291.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2004 Oct;64(4):452-61. doi: 10.1111/j.1399-0039.2004.00291.x.