PMID- 15361129
OWN - NLM
STAT- MEDLINE
DCOM- 20050224
LR  - 20040913
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 64
IP  - 4
DP  - 2004 Oct
TI  - Identification of two new HLA alleles: B*3546* and B*5611*. How reliable are the 
      published HLA-B intron 2 sequences?
PG  - 500-5
AB  - Polymerase chain reaction-sequence-specific primer (PCR-SSP) typing for human 
      leukocyte antigen (HLA)-B in a male 25-year-old Caucasian individual of Iranian 
      origin and in a 42-year-old German Caucasian bone marrow donor revealed reaction 
      patterns that did not agree with any known HLA specificity, thus suggesting in 
      both cases the existence of a novel allele. Sequence-based typing (SBT) after 
      allelic separation revealed the sequences of the new alleles HLA-B*5611 and 
      B*3546. The sequence patterns of both new alleles might have been generated as 
      the results of double crossing over, possibly over several generations. During 
      the analysis of the HLA-B*3546 intron 2 sequence for possible crossing over 
      points, a base insert, an additional G after position 700, was found. This insert 
      was analyzed using SBT and PCR-SSP and was found to be present not only in all 
      samples carrying B*35, but also in all HLA-B specificities tested. It appears 
      that all known HLA-B alleles may contain a G insert at position 700 of intron 2, 
      and that the published intron 2 sequence alignments of the HLA-B locus may 
      contain errors at this position.
FAU - Czachurski, D
AU  - Czachurski D
AD  - Department of Transplantation Immunology, Institute of Immunology, University of 
      Heidelberg, Heidelberg, Germany.
FAU - Scherer, S
AU  - Scherer S
FAU - Gehrke, S
AU  - Gehrke S
FAU - Laux, G
AU  - Laux G
FAU - Opelz, G
AU  - Opelz G
FAU - Mytilineos, J
AU  - Mytilineos J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (DNA Primers)
RN  - 0 (HLA-B Antigens)
SB  - IM
MH  - Adult
MH  - *Alleles
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - Exons/*genetics
MH  - Germany
MH  - HLA-B Antigens/*genetics
MH  - Humans
MH  - Iran
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - *Polymorphism, Single-Stranded Conformational
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
EDAT- 2004/09/14 05:00
MHDA- 2005/02/25 09:00
CRDT- 2004/09/14 05:00
PHST- 2004/09/14 05:00 [pubmed]
PHST- 2005/02/25 09:00 [medline]
PHST- 2004/09/14 05:00 [entrez]
AID - TAN294 [pii]
AID - 10.1111/j.1399-0039.2004.00294.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2004 Oct;64(4):500-5. doi: 10.1111/j.1399-0039.2004.00294.x.