PMID- 15361364
OWN - NLM
STAT- MEDLINE
DCOM- 20050218
LR  - 20171116
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 32
IP  - 1
DP  - 2005 Jan
TI  - Pleiomorphic adenoma gene-like-2, a zinc finger protein, transactivates the 
      surfactant protein-C promoter.
PG  - 35-43
AB  - Expression of surfactant protein (SP)-C occurs principally in type II pneumocytes 
      located in the distal lung alveolae. SP-C expression is thought to be primarily 
      regulated by thyroid transcription factor (TTF)-1 and its associated proteins 
      interacting with a previously defined promoter region between -197 and -158 in 
      mice. We screened a human lung cDNA library using a modified yeast one-hybrid 
      system and identified pleiomorphic adenoma gene-like (PLAGL)-2, a ubiquitously 
      expressed zinc finger protein, as a transfactor of the SP-C promoter. The PLAGL2 
      DNA-binding site was located in the SP-C promoter proximal region close to the 
      TTF-1 sites. This site was demonstrated to be functional by use of 
      electrophoresis mobility shift assay, mutagenesis analysis, and transfection 
      studies. PLAGL2 bound to DNA via its N-terminus zinc fingers and activated the 
      SP-C promoter in a TTF-1-independent manner. Both human and mouse SP-C promoters, 
      but not the SP-B promoter, could be activated by PLAGL2 in transfected human 
      embryonic kidney-293 (HEK293) cells as well as in murine type II (MLE12) cells. 
      The expression of PLAGL2 in isolated human embryonic lung type II cells and its 
      transactivation activity on the SP-C promoter suggest that PLAGL2 may modulate 
      SP-C expression during lung development.
FAU - Yang, Meng-Chun W
AU  - Yang MC
AD  - Department of Internal Medicine, Pulmonary and Critical Care Medicine, The 
      University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 
      75390-9034, USA.
FAU - Weissler, Jonathan C
AU  - Weissler JC
FAU - Terada, Lance S
AU  - Terada LS
FAU - Deng, Feitao
AU  - Deng F
FAU - Yang, Yih-Sheng
AU  - Yang YS
LA  - eng
GR  - R01HL63525/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040910
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NKX2-1 protein, human)
RN  - 0 (Nkx2-1 protein, mouse)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PLAGL2 protein, human)
RN  - 0 (Pulmonary Surfactants)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Thyroid Nuclear Factor 1)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Electrophoretic Mobility Shift Assay
MH  - Genes, Reporter/genetics
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Proteins
MH  - *Promoter Regions, Genetic
MH  - Pulmonary Surfactants/*metabolism
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - Regulatory Sequences, Nucleic Acid/*genetics
MH  - Thyroid Nuclear Factor 1
MH  - Transcription Factors/genetics/*metabolism
MH  - Yeasts/metabolism
EDAT- 2004/09/14 05:00
MHDA- 2005/02/19 09:00
CRDT- 2004/09/14 05:00
PHST- 2004/09/14 05:00 [pubmed]
PHST- 2005/02/19 09:00 [medline]
PHST- 2004/09/14 05:00 [entrez]
AID - 2003-0422OC [pii]
AID - 10.1165/rcmb.2003-0422OC [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 2005 Jan;32(1):35-43. doi: 10.1165/rcmb.2003-0422OC. 
      Epub 2004 Sep 10.