PMID- 15361655
OWN - NLM
STAT- MEDLINE
DCOM- 20050307
LR  - 20190902
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 290
DP  - 2005
TI  - Cytogenetic analysis of cell lines.
PG  - 51-70
AB  - Cytogenetic analysis forms an essential part of characterizing and identifying 
      cell lines, in particular those established from tumors. In addition, karyotypic 
      analysis can be used to distinguish individual subclones and to monitor 
      stability. This chapter describes basic cytogenetic procedures suited to cells in 
      continuous culture. The provision of unlimited material by cell lines encourages 
      an heuristic approach to harvesting and hypotonic treatments to yield metaphase 
      chromosome slide preparations of improved quality suitable for subsequent banding 
      and fluorescence in situ hybridization (FISH) analysis. The experience of the 
      writers with more than 500 different cell lines has shown that no single 
      hypotonic harvesting protocol is adequate to consistently deliver satisfactory 
      chromosome preparations. Thus, evidence-based protocols are described for 
      hypotonic harvesting, rapid G-banding, and FISH analysis of cell cultures to 
      allow troubleshooting and fine-tuning to suit the requirements of individual cell 
      lines.
FAU - MacLeod, Roderick A F
AU  - MacLeod RA
AD  - DSMZ-Department of Human and Animal Cell Cultures, German Collection of 
      Microorganisms and Cell Cultures, Braunschweig, Germany.
FAU - Drexler, Hans G
AU  - Drexler HG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Cell Line
MH  - Chromosome Banding
MH  - *Cytogenetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
EDAT- 2004/09/14 05:00
MHDA- 2005/03/08 09:00
CRDT- 2004/09/14 05:00
PHST- 2004/09/14 05:00 [pubmed]
PHST- 2005/03/08 09:00 [medline]
PHST- 2004/09/14 05:00 [entrez]
AID - 1-59259-838-2:051 [pii]
AID - 10.1385/1-59259-838-2:051 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2005;290:51-70. doi: 10.1385/1-59259-838-2:051.