PMID- 15362033
OWN - NLM
STAT- MEDLINE
DCOM- 20041019
LR  - 20190707
IS  - 0016-5085 (Print)
IS  - 0016-5085 (Linking)
VI  - 127
IP  - 3
DP  - 2004 Sep
TI  - Analysis of hepatitis C virus quasispecies transmission and evolution in patients 
      infected through blood transfusion.
PG  - 764-76
AB  - BACKGROUND & AIMS: Studies on hepatitis C virus (HCV) quasispecies dynamics in 
      the natural course of infection are rare owing to difficulties in obtaining 
      samples from the early phase of infection. METHODS: We studied 15 patients from 
      the Transfusion-Transmitted Viruses Study who seroconverted to anti-HCV after 
      receiving infected blood. Follow-up serum samples were collected every 2-3 weeks 
      for 6 months, at 10 months, and at 11-16 years. Viral quasispecies in the second 
      envelope hypervariable region 1 (E2/HVR1) and 5' untranslated region (5'UTR) were 
      analyzed with single-strand conformation polymorphism (SSCP) and heteroduplex 
      mobility assay (HMA). RESULTS: Seven patients cleared infection within 7-24 weeks 
      (mean, 14.0 wk) and 3 patients eventually became anti-HCV negative. In 6 patients 
      with resolving hepatitis the SSCP band pattern remained stable, whereas in one 
      patient minor changes appeared before clearance. In contrast, in all 8 patients 
      progressing to chronicity, major changes in the E2/HVR1 quasispecies developed at 
      8-22 weeks (mean, 13.1 wk). Shannon entropy and medium mobility shift values 
      derived from HMA gels remained stable in patients with resolving hepatitis but 
      changed in those who developed chronic infection. Only 2 patients showed minor 
      changes in 5'UTR. A decrease in E2/HVR1 complexity at the time of transmission 
      (bottleneck) was found in 5 patients altogether. CONCLUSIONS: Changes in E2/HVR1 
      quasispecies 8-22 weeks after infection, likely caused by mounting immune 
      pressure, were predictive of ensuing chronic infection, whereas stability was 
      associated with resolution. Our study also showed that composition of HCV 
      quasispecies may be preserved during transmission from host to host.
FAU - Laskus, Tomasz
AU  - Laskus T
AD  - Department of Medicine, Mayo Clinic Scottsdale, Scottsdale, Arizona, USA.
FAU - Wilkinson, Jeffrey
AU  - Wilkinson J
FAU - Gallegos-Orozco, Juan F
AU  - Gallegos-Orozco JF
FAU - Radkowski, Marek
AU  - Radkowski M
FAU - Adair, Debra M
AU  - Adair DM
FAU - Nowicki, Marek
AU  - Nowicki M
FAU - Operskalski, Eva
AU  - Operskalski E
FAU - Buskell, Zelma
AU  - Buskell Z
FAU - Seeff, Leonard B
AU  - Seeff LB
FAU - Vargas, Hugo
AU  - Vargas H
FAU - Rakela, Jorge
AU  - Rakela J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Gastroenterology
JT  - Gastroenterology
JID - 0374630
SB  - IM
MH  - Adult
MH  - *Disease Transmission, Infectious
MH  - Evolution, Molecular
MH  - Female
MH  - Hepacivirus/*genetics
MH  - Hepatitis C/transmission/*virology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Species Specificity
MH  - *Transfusion Reaction
EDAT- 2004/09/14 05:00
MHDA- 2004/10/20 09:00
CRDT- 2004/09/14 05:00
PHST- 2004/09/14 05:00 [pubmed]
PHST- 2004/10/20 09:00 [medline]
PHST- 2004/09/14 05:00 [entrez]
AID - S0016508504010248 [pii]
AID - 10.1053/j.gastro.2004.06.005 [doi]
PST - ppublish
SO  - Gastroenterology. 2004 Sep;127(3):764-76. doi: 10.1053/j.gastro.2004.06.005.