PMID- 15363038
OWN - NLM
STAT- MEDLINE
DCOM- 20050203
LR  - 20181130
IS  - 1320-5463 (Print)
IS  - 1320-5463 (Linking)
VI  - 54
IP  - 9
DP  - 2004 Sep
TI  - GM-CSF activates RhoA, integrin and MMP expression in human monocytic cells.
PG  - 693-702
AB  - Monocyte migration is one of the key events occurring in the early stage of 
      atherosclerosis. This process includes monocytic adhesion to and penetration 
      through the arterial intima. In such an environment, many factors stimulate the 
      monocytes to enhance integrin activation and extracellular matrix degradation. To 
      investigate the coordinative operation of these two events in relation to 
      monocyte migration, we paid particular attention to the effects of 
      granulocyte-macrophage colony-stimulating factor (GM-CSF) on monocytes in terms 
      of RhoA activation and matrix metalloproteinase (MMP) expression. RhoA and 
      integrin clustering were activated by GM-CSF, monocyte chemoattractant protein-1 
      (MCP-1) and platelet-derived growth factor-BB (PDGF-BB) in human monocytic cell 
      lines. Furthermore, enhancement of migration was observed with stimulation by 
      MCP-1 and PDGF-BB. Granulocyte-macrophage colony-stimulating factor did not 
      enhance the migration, even though it activated RhoA and integrin. However, 
      GM-CSF is known to stimulate monocytes to express MCP-1, suggesting the presence 
      of an indirect mechanism for GM-CSF-mediated migratory activity. In contrast, 
      only GM-CSF enhanced the expression of MMP-1 and MMP-9. These results provide 
      evidence that GM-CSF has multiple functions enhancing monocytic migration via 
      RhoA and integrin activation, and via MMP expression.
FAU - Kohno, Yukari
AU  - Kohno Y
AD  - Department of Pathology and Cell Biology, School of Medicine, University of 
      Occupational and Environmental Health, Kitakyushu, Japan.
FAU - Tanimoto, Akihide
AU  - Tanimoto A
FAU - Cirathaworn, Cintana
AU  - Cirathaworn C
FAU - Shimajiri, Shohei
AU  - Shimajiri S
FAU - Tawara, Akihiko
AU  - Tawara A
FAU - Sasaguri, Yasuyuki
AU  - Sasaguri Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Integrins)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (RNA, Messenger)
RN  - 124671-05-2 (RHOA protein, human)
RN  - 1B56C968OA (Becaplermin)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Becaplermin
MH  - Blotting, Northern
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - Chemokine CCL2/pharmacology
MH  - Genes, Reporter
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology/physiology
MH  - Humans
MH  - Integrins/*biosynthesis
MH  - Luciferases
MH  - Matrix Metalloproteinases/*biosynthesis/genetics
MH  - Monocytes/*drug effects/metabolism
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Proto-Oncogene Proteins c-sis
MH  - RNA, Messenger/metabolism
MH  - rhoA GTP-Binding Protein/*biosynthesis
EDAT- 2004/09/15 05:00
MHDA- 2005/02/04 09:00
CRDT- 2004/09/15 05:00
PHST- 2004/09/15 05:00 [pubmed]
PHST- 2005/02/04 09:00 [medline]
PHST- 2004/09/15 05:00 [entrez]
AID - PIN1682 [pii]
AID - 10.1111/j.1440-1827.2004.01682.x [doi]
PST - ppublish
SO  - Pathol Int. 2004 Sep;54(9):693-702. doi: 10.1111/j.1440-1827.2004.01682.x.