PMID- 15363096
OWN - NLM
STAT- MEDLINE
DCOM- 20050608
LR  - 20181113
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 5
DP  - 2004 Sep 13
TI  - An integrated 4249 marker FISH/RH map of the canine genome.
PG  - 65
AB  - BACKGROUND: The 156 breeds of dog recognized by the American Kennel Club offer a 
      unique opportunity to map genes important in genetic variation. Each breed 
      features a defining constellation of morphological and behavioral traits, often 
      generated by deliberate crossing of closely related individuals, leading to a 
      high rate of genetic disease in many breeds. Understanding the genetic basis of 
      both phenotypic variation and disease susceptibility in the dog provides new ways 
      in which to dissect the genetics of human health and biology. RESULTS: To 
      facilitate both genetic mapping and cloning efforts, we have constructed an 
      integrated canine genome map that is both dense and accurate. The resulting 
      resource encompasses 4249 markers, and was constructed using the RHDF5000-2 whole 
      genome radiation hybrid panel. The radiation hybrid (RH) map features a density 
      of one marker every 900 Kb and contains 1760 bacterial artificial chromosome 
      clones (BACs) localized to 1423 unique positions, 851 of which have also been 
      mapped by fluorescence in situ hybridization (FISH). The two data sets show 
      excellent concordance. Excluding the Y chromosome, the map features an RH/FISH 
      mapped BAC every 3.5 Mb and an RH mapped BAC-end, on average, every 2 Mb. For 
      2233 markers, the orthologous human genes have been established, allowing the 
      identification of 79 conserved segments (CS) between the dog and human genomes, 
      dramatically extending the length of most previously described CS. CONCLUSIONS: 
      These results provide a necessary resource for the canine genome mapping 
      community to undertake positional cloning experiments and provide new insights 
      into the comparative canine-human genome maps.
FAU - Breen, Matthew
AU  - Breen M
AD  - Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 
      North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, 
      USA. matthew_breen@ncsu.edu
FAU - Hitte, Christophe
AU  - Hitte C
FAU - Lorentzen, Travis D
AU  - Lorentzen TD
FAU - Thomas, Rachael
AU  - Thomas R
FAU - Cadieu, Edouard
AU  - Cadieu E
FAU - Sabacan, Leah
AU  - Sabacan L
FAU - Scott, Allyson
AU  - Scott A
FAU - Evanno, Gwenaelle
AU  - Evanno G
FAU - Parker, Heidi G
AU  - Parker HG
FAU - Kirkness, Ewen F
AU  - Kirkness EF
FAU - Hudson, Ruth
AU  - Hudson R
FAU - Guyon, Richard
AU  - Guyon R
FAU - Mahairas, Gregory G
AU  - Mahairas GG
FAU - Gelfenbeyn, Boris
AU  - Gelfenbeyn B
FAU - Fraser, Claire M
AU  - Fraser CM
FAU - Andre, Catherine
AU  - Andre C
FAU - Galibert, Francis
AU  - Galibert F
FAU - Ostrander, Elaine A
AU  - Ostrander EA
LA  - eng
GR  - K05 CA090754/CA/NCI NIH HHS/United States
GR  - R01 CA092167/CA/NCI NIH HHS/United States
GR  - R01CA-92167/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040913
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping/methods
MH  - Chromosomes, Artificial, Bacterial
MH  - Dogs/*genetics
MH  - Genetic Markers
MH  - *Genome
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microsatellite Repeats
MH  - Radiation Hybrid Mapping
MH  - Synteny
PMC - PMC520820
EDAT- 2004/09/15 05:00
MHDA- 2005/06/09 09:00
PMCR- 2004/09/13
CRDT- 2004/09/15 05:00
PHST- 2004/08/23 00:00 [received]
PHST- 2004/09/13 00:00 [accepted]
PHST- 2004/09/15 05:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2004/09/15 05:00 [entrez]
PHST- 2004/09/13 00:00 [pmc-release]
AID - 1471-2164-5-65 [pii]
AID - 10.1186/1471-2164-5-65 [doi]
PST - epublish
SO  - BMC Genomics. 2004 Sep 13;5:65. doi: 10.1186/1471-2164-5-65.