PMID- 15363120
OWN - NLM
STAT- MEDLINE
DCOM- 20050126
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 12
IP  - 4
DP  - 2004 Aug
TI  - Coexistence of tetrasomy 8 and trisomy 8 in acute promyelocytic leukemia (AML-M3) 
      with t(15;17)(q22;q12).
PG  - 406-10
AB  - This study was purposed to characterize the first case of acute promyelocitic 
      leukemia (AML-M(3a)) with t(15;17), trisomy 8 and tetrasomy 8, and explore its 
      characteristics of morphology, cytogenetics, molecular biology, immunology and 
      clinical features. Morphological changes of peripheral blood and bone marrow 
      smears were observed under microscope. Chromosome specimen was prepared by 24 h 
      short-term culture of bone marrow cell, RHG-banding technique was used for 
      karyotypic analysis. PML-RARa fusion gene transcript was detected by 
      nested-reverse transcription-polymerase chain reaction (nested RT-PCR). 
      Interphase fluorescence in situ hybridization (FISH) using chromosome 8 
      centromere specific probe were carried out to detect abnormal numbers of 
      chromosome 8. Immunophenotypic analysis was performed by flow cytometry. The 
      results showed that peripheral blood smear revealed 65% promyelocyte, and bone 
      marrow aspirate was hypercellular with 72.4% promyelocyte, moderately basophilic 
      cytoplasm with numerous azurophilic granules. Karyotype analysis demonstrated 48, 
      XY, +8, +8, t(15;17)(q22;q12) [16]/47, XY, +8, t(15;17)(q22;q12) [3]/46, XY, 
      t(15;17)(q22;q12) [1]. RT-PCR assay revealed PML-RARa fusion gene transcript (+). 
      FISH showed that the percentages of cells exhibiting 1, 2, 3, 4, 5, 6 green 
      fluorescence signals were 0.5, 7, 19, 55, 18 and 0.5, respectively. This 
      confirmed the presence of tetrasomy 8 and trisomy 8 and also revealed a low 
      percentage of a pentasomy 8 clone. Immunophenotypes of the blasts displayed that 
      CD13 (96.2%), CD33 (55.9%), CYMPO (93.5%) were positive. All the lymphoid markers 
      tested were negative. The patient survival time was just 10 days. It is concluded 
      that tetrasomy 8 is secondary cytogenetic event after t(15;17) in this case. It 
      may be a consequence of clonal evolution of trisomy 8. t(15;17) AML-M(3) with 
      tetrasomy 8 heralds a poor prognosis.
FAU - Wang, Hui-Ping
AU  - Wang HP
AD  - Department of Hematology, The Second Hospital of Shanxi Medical University, 
      Taiyuan 030001, China.
FAU - Li, Guo-Xia
AU  - Li GX
FAU - Qiao, Zhen-Hua
AU  - Qiao ZH
FAU - Ren, Wen-Ying
AU  - Ren WY
FAU - Wang, Hong-Wei
AU  - Wang HW
LA  - eng
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (RNA, Messenger)
RN  - 0 (promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein)
SB  - IM
MH  - *Chromosomes, Human, Pair 15
MH  - *Chromosomes, Human, Pair 17
MH  - *Chromosomes, Human, Pair 8
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Promyelocytic, Acute/*genetics
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Proteins/*genetics
MH  - Oncogene Proteins, Fusion/*genetics
MH  - RNA, Messenger/analysis
MH  - *Translocation, Genetic
MH  - *Trisomy
EDAT- 2004/09/15 05:00
MHDA- 2005/01/27 09:00
CRDT- 2004/09/15 05:00
PHST- 2004/09/15 05:00 [pubmed]
PHST- 2005/01/27 09:00 [medline]
PHST- 2004/09/15 05:00 [entrez]
AID - 1009-2137(2004)04-0406-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2004 Aug;12(4):406-10.