PMID- 15364270
OWN - NLM
STAT- MEDLINE
DCOM- 20041210
LR  - 20181130
IS  - 1386-6532 (Print)
IS  - 1386-6532 (Linking)
VI  - 31
IP  - 2
DP  - 2004 Oct
TI  - Influenza circulating strains in Argentina exhibit differential induction of 
      cytotoxicity and caspase-3 in vitro.
PG  - 134-9
AB  - BACKGROUND: Human influenza infections are a significant cause of morbidity 
      worldwide. Though damage to the respiratory epithelium and has been related to 
      apoptosis, which occurs subsequent to influenza virus infection, little 
      information is available regarding cell cytotoxicity of human strains. OBJECTIVE: 
      To study cytotoxicity performed in vitro by various circulating strains in 
      Argentina. The study sample consisted of three vaccine strains (H1N1, H3N2, and 
      B) administered during 1999-2000 in South America and three strains isolated from 
      clinical samples, one, NAC (H1N1) obtained from an adult inpatient with human 
      pneumonia; and the other two (T) and (T2) (H3N2) with influenza syndrome. Viral 
      antigen was detected by an immunofluorescence test, conducted prior to viral 
      isolation in MDCK cells. Strains were subtyped by the hemmaglutination inhibition 
      test. Cytotoxic properties were determined by lactate dehydrogenase reaction 
      (LDH), crystal violet staining and Hoechst staining. Caspase-3 activity, 
      morphological changes of apoptosis, and viral yields were measured in MDCK 
      infected cells. RESULTS AND CONCLUSIONS: Cells infected by each of the strains 
      exhibited apoptosis morphology by Hoechst staining and caspase-3 activity was 
      high for both H1N1 strains. Further, high levels of LDH activity were detected 
      for NAC and H3N2 strains tested, indicating the possible role of different viral 
      proteins or functions on cell cytotoxicity. The NAC strain, isolated from human 
      pneumonia and antigenically related to A/New Caledonia /20/99 (H1N1), was the 
      highest cytotoxic strain and an excellent inducer of caspase-3 activity. In turn, 
      no parameter was related to different viral yields. We conclude that human 
      strains studied in this paper may be useful tools in the characterization of 
      molecular determinants involved in viral cytopathogenicity.
FAU - Mersich, S E
AU  - Mersich SE
AD  - Department of Biochemistry, Laboratory of Virology, School of Science, University 
      of Buenos Aires, Buenos Aires, Argentina.
FAU - Baumeister, E G
AU  - Baumeister EG
FAU - Riva, D
AU  - Riva D
FAU - Lewis, A P
AU  - Lewis AP
FAU - Cadario, M E
AU  - Cadario ME
FAU - Pontoriero, A V
AU  - Pontoriero AV
FAU - Savy, V L
AU  - Savy VL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Clin Virol
JT  - Journal of clinical virology : the official publication of the Pan American 
      Society for Clinical Virology
JID - 9815671
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Argentina
MH  - Caspase 3
MH  - Caspases/biosynthesis
MH  - Cell Line
MH  - Cytotoxicity, Immunologic
MH  - Dogs
MH  - Enzyme Induction
MH  - Humans
MH  - In Vitro Techniques
MH  - Influenza A virus/*isolation & purification/*pathogenicity
MH  - Influenza B virus/*isolation & purification/*pathogenicity
MH  - Influenza, Human/*virology
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Species Specificity
MH  - Virus Cultivation
EDAT- 2004/09/15 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/09/15 05:00
PHST- 2003/12/10 00:00 [revised]
PHST- 2004/01/10 00:00 [accepted]
PHST- 2004/09/15 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/09/15 05:00 [entrez]
AID - S1386653204000447 [pii]
AID - 10.1016/j.jcv.2004.01.004 [doi]
PST - ppublish
SO  - J Clin Virol. 2004 Oct;31(2):134-9. doi: 10.1016/j.jcv.2004.01.004.