PMID- 15366744
OWN - NLM
STAT- MEDLINE
DCOM- 20040930
LR  - 20190827
IS  - 0364-2313 (Print)
IS  - 0364-2313 (Linking)
VI  - 28
IP  - 6
DP  - 2004 Jun
TI  - Impact of circulating free tumor cells in the peripheral blood of colorectal 
      cancer patients during laparoscopic surgery.
PG  - 552-7
AB  - Despite widespread use of laparoscopic surgery for colorectal operations, its 
      application for curative resection of colorectal cancer is still controversial. 
      One of the major concerns is the impact of the laparoscopic procedure on 
      dissemination of tumor cells. The main purpose of this study was to investigate 
      the impact of laparoscopic surgery on circulating tumor cells in colorectal 
      cancer patients. Quantitation of circulating free tumor cells (FTCs) was 
      performed preoperatively, during the operation, and 14 days later by means of 
      real-time quantitative reverse transcriptasepolymerase chain reaction (RT-PCR) 
      targeting guanylyl cyclase C (GCC) mRNA in 42 colorectal cancer patients 
      undergoing laparoscopic resections. Despite an increasing trend of FTC detection 
      in patients with advancing stage, there is no significant difference in the 
      preoperative FTC level by disease stage. No elevation in FTC level was found 
      during the laparoscopic procedure in most patients compared with their 
      preoperative FTC value. Patients with a persistently high FTC load [per nucleated 
      blood cells (NBCs)] (> 10(2) FTCs/10(6) NBCs) 2 weeks postoperatively portends a 
      poor prognosis regarding disease recurrence and tumor-related mortality when 
      compared to those with an undetectable or low FTC load (< or = 10(2) FTCs/10(6) 
      NBCs). We concluded that the laparoscopic procedure itself had no significantly 
      deleterious effect on circulating FTCs and that the detection of FTCs by 
      real-time qRT-PCR might be of clinical importance during the postoperative 
      follow-up for colorectal cancer patients.
FAU - Chen, Wei-Shone
AU  - Chen WS
AD  - Division of Colorectal Surgery, Taipei Veterans General Hospital, National 
      Yang-Ming University, Taipei, Taiwan. wschen@vghtpe.gov.tw
FAU - Chung, Ming-Yi
AU  - Chung MY
FAU - Liu, Jin-Hwang
AU  - Liu JH
FAU - Liu, Jacqueline Ming
AU  - Liu JM
FAU - Lin, Jen-Kou
AU  - Lin JK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Surg
JT  - World journal of surgery
JID - 7704052
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Colorectal Neoplasms/*blood/*surgery
MH  - Disease-Free Survival
MH  - Female
MH  - Humans
MH  - *Laparoscopy
MH  - Male
MH  - Middle Aged
MH  - *Neoplastic Cells, Circulating
MH  - Postoperative Period
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2004/09/16 05:00
MHDA- 2004/10/01 05:00
CRDT- 2004/09/16 05:00
PHST- 2004/09/16 05:00 [pubmed]
PHST- 2004/10/01 05:00 [medline]
PHST- 2004/09/16 05:00 [entrez]
AID - 10.1007/s00268-004-7276-9 [doi]
PST - ppublish
SO  - World J Surg. 2004 Jun;28(6):552-7. doi: 10.1007/s00268-004-7276-9.