PMID- 15367644
OWN - NLM
STAT- MEDLINE
DCOM- 20041021
LR  - 20191210
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 78
IP  - 19
DP  - 2004 Oct
TI  - Cell fusion activities of Hantaan virus envelope glycoproteins.
PG  - 10776-82
AB  - Hantaan virus (HTNV)-infected Vero E6 cells undergo cell fusion with both 
      infected and uninfected cells under low-pH conditions. Flow cytometry and 
      fluorescence microscopy of HTNV-infected Vero E6 cells showed that envelope 
      glycoproteins (GPs) were located both on the cell surface and in the cytoplasm. 
      Neutralizing monoclonal antibodies (MAbs) against the G1 and G2 envelope GPs 
      inhibited cell fusion, whereas nonneutralizing MAbs against G1 or G2 and MAbs 
      against the nucleocapsid protein (NP) did not. Transfected Vero E6 cells that 
      expressed GPs but not those that expressed NP fused and formed syncytia. These 
      results indicate that HTNV GPs act as fusogens at the cell surface. No fusion 
      activity was observed either in infected Vero cells that were passaged more than 
      150 times or in BHK-21 cells, although GPs appeared to localize to the cell 
      surface. This variability in fusion induction suggests the involvement of host 
      cell factors in the process of cell membrane fusion.
FAU - Ogino, Michiko
AU  - Ogino M
AD  - Institute for Animal Experimentation, Graduate School of Medicine, Hokkaido 
      University, Sapporo, Japan.
FAU - Yoshimatsu, Kumiko
AU  - Yoshimatsu K
FAU - Ebihara, Hideki
AU  - Ebihara H
FAU - Araki, Koichi
AU  - Araki K
FAU - Lee, Byoung-Hee
AU  - Lee BH
FAU - Okumura, Megumi
AU  - Okumura M
FAU - Arikawa, Jiro
AU  - Arikawa J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Fusion Proteins)
RN  - 0 (glycoprotein G2, Hantavirus)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Antibodies, Viral/metabolism
MH  - *Cell Fusion
MH  - Cell Membrane/chemistry
MH  - Chlorocebus aethiops
MH  - Cytoplasm/chemistry
MH  - Epitopes/immunology
MH  - Flow Cytometry
MH  - Giant Cells/cytology/virology
MH  - Glycoproteins/*physiology
MH  - Hantaan virus/pathogenicity/*physiology
MH  - Hydrogen-Ion Concentration
MH  - Microscopy, Fluorescence
MH  - Vero Cells
MH  - Viral Envelope Proteins/analysis/*metabolism/physiology
MH  - Viral Fusion Proteins/*metabolism
PMC - PMC516380
EDAT- 2004/09/16 05:00
MHDA- 2004/10/22 09:00
PMCR- 2004/10/01
CRDT- 2004/09/16 05:00
PHST- 2004/09/16 05:00 [pubmed]
PHST- 2004/10/22 09:00 [medline]
PHST- 2004/09/16 05:00 [entrez]
PHST- 2004/10/01 00:00 [pmc-release]
AID - 78/19/10776 [pii]
AID - 0192-04 [pii]
AID - 10.1128/JVI.78.19.10776-10782.2004 [doi]
PST - ppublish
SO  - J Virol. 2004 Oct;78(19):10776-82. doi: 10.1128/JVI.78.19.10776-10782.2004.