PMID- 15371170
OWN - NLM
STAT- MEDLINE
DCOM- 20050215
LR  - 20131121
IS  - 1091-5818 (Print)
IS  - 1091-5818 (Linking)
VI  - 23
IP  - 4
DP  - 2004 Jul-Aug
TI  - Organophosphorus compound-induced delayed neurotoxicity in white leghorn hens 
      assessed by Fluoro-Jade.
PG  - 259-66
AB  - Certain organophosphorus (OP) compounds can induce a delayed neuropathy, termed 
      OPIDN, that involves central and peripheral nervous system axons, terminals, and 
      perikarya. Historically, OPIDN has been characterized by staining neural sections 
      with silver or hematoxylin and eosin (H and E). This study utilized a novel 
      staining method, Fluoro-Jade, for evaluating the distribution and extent of OPIDN 
      in the central nervous system of hens. Results were then compared to synoptically 
      sectioned and stained H and E preparations. White Leghorn hens were injected with 
      phenyl saligenin phosphate (PSP, 2.5 mg/kg, intramuscular [im]), triphenyl 
      phosphite (TPPi, 500 mg/kg, subcutaneous [sc]), or dimethyl sulfoxide vehicle 
      (DMSO, 0.5 ml/kg, im or sc) and evaluated clinically for signs of neurological 
      dysfunction associated with OPIDN. Hens were sacrificed 7, 14, and 21 days post 
      dosing. Brains and spinal cords were removed immediately following sacrifice, 
      fixed in formalin, and embedded in paraffin. Microtome-cut sections (7 micro m) 
      were then stained with Fluoro-Jade (0.001%, w/v) or H&E. Staining with 
      Fluoro-Jade revealed time-dependent degeneration of nerve fibers and terminals 
      (with PSP and TPPi), or cell bodies (with TPPi) in lamina VII, spinocerebellar, 
      and medial pontine-spinal tracts of the lumbar spinal cord, in white matter and 
      mossy fibers of foliae I-V and IX of the cerebellum, and in medullary, pontine, 
      and midbrain nuclei and paleostriatal fibers surrounding the optic tract. 
      TPPi-induced degeneration was more extensive than that induced by PSP and 
      affected additional cerebellar folia, medullary, pontine, midbrain, and forebrain 
      nuclei and fiber tracts. H&E-stained sections revealed fewer sites of 
      neurodegeneration when compared to Fluoro-Jade. These results demonstrate that 
      Fluoro-Jade is a sensitive method for staining neural tissue affected by OPIDN.
FAU - Carlson, Kent
AU  - Carlson K
AD  - Laboratory for Neurotoxicity Studies, Virginia Tech, Virginia-Maryland Regional 
      College of Veterinary Medicine, Blacksburg, Virginia 24061, USA.
FAU - Ehrich, Marion
AU  - Ehrich M
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Int J Toxicol
JT  - International journal of toxicology
JID - 9708436
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Organic Chemicals)
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (fluoro jade)
SB  - IM
MH  - Animals
MH  - Brain/*drug effects/pathology
MH  - *Chickens
MH  - Female
MH  - Fluoresceins
MH  - *Fluorescent Dyes
MH  - Organic Chemicals
MH  - Organophosphorus Compounds/*toxicity
MH  - Spinal Cord/*drug effects/pathology
MH  - Staining and Labeling/*methods
MH  - Time Factors
EDAT- 2004/09/17 05:00
MHDA- 2005/02/16 09:00
CRDT- 2004/09/17 05:00
PHST- 2004/09/17 05:00 [pubmed]
PHST- 2005/02/16 09:00 [medline]
PHST- 2004/09/17 05:00 [entrez]
AID - H1LRBWL74DKW4013 [pii]
AID - 10.1080/10915810490504968 [doi]
PST - ppublish
SO  - Int J Toxicol. 2004 Jul-Aug;23(4):259-66. doi: 10.1080/10915810490504968.