PMID- 1537297
OWN - NLM
STAT- MEDLINE
DCOM- 19920402
LR  - 20071114
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 130
IP  - 3
DP  - 1992 Mar
TI  - Expression of tumor necrosis factor-alpha in the rat ovary.
PG  - 1359-64
AB  - Tumor necrosis factor-alpha (TNF-alpha) messenger RNA (mRNA) in rat ovaries was 
      detected by Northern blot analysis and by reverse transcription-polymerase chain 
      reaction (RT-PCR). Northern blot analysis of total cellular RNA (tcRNA) isolated 
      from adult rat ovaries showed a single distinct band at approximately 2 
      kilobases, while rat white blood cells, used as control, also showed a second 
      lower band at approximately 0.5 kilobases. TNF-alpha expression in ovaries was 
      also detected by RT-PCR, using RNA-specific primers. Southern blot analysis of 
      the RT-PCR products showed a single band of the expected 500 base pairs, from the 
      ovary and white blood cells, using either tcRNA or polyA+ RNA. In order to 
      validate the RT-PCR product, it was digested with restriction enzymes, HhaI, 
      HindIII, and XhoI. The results indicate that the ovarian TNF-alpha mRNA does not 
      contain major alterations with respect to the known structure of rat TNF-alpha 
      mRNA. Ovarian RNA was also subjected to PCR without previous RT in order to 
      amplify, if any, contaminating genomic DNA. A single band of approximately 900 
      base pairs was observed, which contained introns 1 and 2 (determined by 
      restriction enzyme digestion). In summary, these results indicate that rat 
      ovaries contain TNF-alpha mRNA which makes it a likely source of local TNF-alpha 
      secretion. The possibility exists that ovarian cells and/or macrophages are the 
      source of the ovarian TNF-alpha mRNA.
FAU - Sancho-Tello, M
AU  - Sancho-Tello M
AD  - Department of Physiology, University of Kansas Medical Center, Kansas 66103.
FAU - Perez-Roger, I
AU  - Perez-Roger I
FAU - Imakawa, K
AU  - Imakawa K
FAU - Tilzer, L
AU  - Tilzer L
FAU - Terranova, P F
AU  - Terranova PF
LA  - eng
GR  - CA-50616/CA/NCI NIH HHS/United States
GR  - HD-02528/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-49-2 (DNA)
SB  - IM
GS  - TNF-&agr;
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - DNA/genetics
MH  - Female
MH  - Gene Expression/*genetics
MH  - Molecular Sequence Data
MH  - Ovary/*chemistry/metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis/genetics
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/analysis/*genetics/metabolism
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
AID - 10.1210/endo.130.3.1537297 [doi]
PST - ppublish
SO  - Endocrinology. 1992 Mar;130(3):1359-64. doi: 10.1210/endo.130.3.1537297.