PMID- 15373466
OWN - NLM
STAT- MEDLINE
DCOM- 20060502
LR  - 20191210
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 76
IP  - 17
DP  - 2004 Sep 1
TI  - Signaling aptamer/protein binding by a molecular light switch complex.
PG  - 5230-5
AB  - A novel method of signaling aptamer/protein binding for aptamer-based protein 
      detection has been developed using a molecular light switch complex, 
      [Ru(phen)2(dppz)]2+. The method takes advantage of the sensitive luminescence 
      signal change of [Ru(phen)2(dppz)]2+ intercalating to the aptamer upon 
      protein/aptamer binding. A 37-nt DNA aptamer against immunoglobulin E (IgE) was 
      first tested as a model system. The luminescence of the [Ru(phen)2(dppz)]2+/IgE 
      aptamer decreased with the increase of IgE. By monitoring the luminescence 
      change, we were able to detect the binding events between the aptamer and IgE for 
      IgE quantitation in homogeneous solutions as well as in serum. The assay was 
      highly selective and sensitive with a detection limit of 100 pM for IgE. This new 
      method is very simple and without the need for the covalent coupling of 
      fluorophores to aptamers. The generalizability of the method was demonstrated by 
      the direct detection of two other proteins, oncoprotein platelet derived growth 
      factor-BB (PDGF-BB) using its DNA aptamer and alpha-thrombin using its RNA 
      aptamer. This new approach is expected to promote the exploitation of 
      aptamer-based biosensors for protein assays in biochemical and biomedical 
      studies.
CI  - Copyright 2004 American Chemical Society
FAU - Jiang, Yaxin
AU  - Jiang Y
AD  - Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080 (China).
FAU - Fang, Xiaohong
AU  - Fang X
FAU - Bai, Chunli
AU  - Bai C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Buffers)
RN  - 0 (Organometallic Compounds)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (Protons)
RN  - 0 (Ru(phen)(2)dipyrido(3,2-a-2',3'-c)phenazine)
RN  - 1B56C968OA (Becaplermin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Aptamers, Nucleotide/*analysis/chemistry
MH  - Becaplermin
MH  - Buffers
MH  - DNA/analysis/chemistry
MH  - Humans
MH  - Immunoglobulin E/analysis/chemistry
MH  - Light
MH  - Luminescent Measurements/*methods
MH  - Organometallic Compounds/*chemistry
MH  - Osmolar Concentration
MH  - Platelet-Derived Growth Factor/analysis/chemistry
MH  - Protein Binding
MH  - Proteins/*analysis/chemistry
MH  - Proto-Oncogene Proteins c-sis
MH  - Protons
MH  - RNA/analysis/chemistry
MH  - Sensitivity and Specificity
MH  - Thrombin/analysis/chemistry
EDAT- 2004/09/18 05:00
MHDA- 2006/05/03 09:00
CRDT- 2004/09/18 05:00
PHST- 2004/09/18 05:00 [pubmed]
PHST- 2006/05/03 09:00 [medline]
PHST- 2004/09/18 05:00 [entrez]
AID - 10.1021/ac049565u [doi]
PST - ppublish
SO  - Anal Chem. 2004 Sep 1;76(17):5230-5. doi: 10.1021/ac049565u.