PMID- 15373581
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20070626
LR  - 20161021
IS  - 0894-203X (Print)
IS  - 0894-203X (Linking)
VI  - 17
IP  - 3
DP  - 2001
TI  - Development of anti-Bw6 reactivity in patients receiving r-GCSF: a preliminary 
      report.
PG  - 63-9
AB  - Recombinant granulocyte colony-stimulating factor (r-GCSF) is used in autologous 
      bone marrow/peripheral blood progenitor cell transplantation (ABMT/PBPC) to 
      shorten the period of neutropenia. As these patients require platelet 
      transfusions, their sera may be monitored for the presence of platelet/human 
      leukocyte antigen (HLA) antibodies. Sera of some patients on r-GCSF (16 
      microg/kg/day) became difficult to evaluate in vitro for the presence of HLA and 
      platelet antibodies because of apparently anomalous reactions in solid phase red 
      cell adherence (SPRCA) assays. SPRCA tests were positive only when platelets were 
      adhered to the polystyrene plates in the presence of glucose; when other simple 
      sugars were used, the patients' sera failed to react. HLA Bw6-positive platelets 
      were more likely than HLA Bw6-negative platelets to be reactive (p <.001). The 
      transfusion of HLA Bw6-positive platelets to patients displaying this in vitro 
      reactivity (positive patients) resulted in a 50 percent lower corrected count 
      increment (CCI) than those given to patients without it (negative patients; p = 
      <.001). When all transfusions were considered, the CCI for those of the positive 
      patients was decreased 73 percent when compared to the control patients (p 
      =.0005). The presence of the antibody also was associated with a twofold increase 
      in the number of platelet transfusions given (p =.0005). ABMT/PBPC patients 
      receiving r-GCSF may develop unexpected reactions on SPRCA antibody screens and 
      poor responses to transfusion of Bw-6-positive platelets.
FAU - Leach, M F
AU  - Leach MF
AD  - Transfusion Service Laboratory, Department of Pathology, Dartmouth- Hitchcock 
      Medical Center, One Medical Center Drive, Lebanon, NH 03756, USA.
FAU - Aubuchon, J P
AU  - Aubuchon JP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Immunohematology
JT  - Immunohematology
JID - 8806387
EDAT- 2004/09/18 05:00
MHDA- 2004/09/18 05:01
CRDT- 2004/09/18 05:00
PHST- 2004/09/18 05:00 [pubmed]
PHST- 2004/09/18 05:01 [medline]
PHST- 2004/09/18 05:00 [entrez]
PST - ppublish
SO  - Immunohematology. 2001;17(3):63-9.