PMID- 15373761
OWN - NLM
STAT- MEDLINE
DCOM- 20050304
LR  - 20111117
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 52
IP  - 3
DP  - 2004 Sep
TI  - HLA-G expression in placenta in relation to HLA-G genotype and polymorphisms.
PG  - 212-7
AB  - PROBLEM: The expression of the non-classical human leukocyte antigen (HLA) class 
      Ib gene, HLA-G, seems to be important at the feto-maternal interface. The HLA-G 
      molecule is almost monomorphic and expressed in both membrane-bound and soluble 
      isoforms. It has been shown to inhibit natural killer cell -mediated lysis and 
      influence cytokine expression. HLA-G gene polymorphism has been linked to 
      differences in gene expression profile of alternatively spliced HLA-G transcripts 
      and levels of specific HLA-G messenger RNA (mRNA) isoforms. Furthermore, aberrant 
      HLA-G expression has been reported in preeclamptic placentas. On this background 
      it is of general interest to further elucidate any associations between HLA-G 
      polymorphism and protein expression. METHODS: We have investigated HLA-G protein 
      expression by immunohistochemistry in HLA-G genotyped placentas from term. HLA-G 
      mRNA expression in preeclamptic placentas and in control placentas was also 
      studied by microarray technology. RESULTS AND CONCLUSIONS: The studies of HLA-G 
      protein expression in term placentas by immunohistochemical analysis showed no 
      clear associations with HLA-G genotypes although this could be because of the 
      very semi-quantitative nature of this technique. However, we found a tendency 
      towards reduction of HLA-G mRNA expression in placentas from preeclamptic cases 
      compared to matched controls with the use of microarray technology.
FAU - Hviid, Thomas Vauvert F
AU  - Hviid TV
AD  - Department of Clinical Biochemistry, Copenhagen University Hospital, 
      Rigshospitalet, Copenhagen O, Denmark. hviid@dadlnet.dk
FAU - Larsen, Lise Grupe
AU  - Larsen LG
FAU - Hoegh, Anne Mette
AU  - Hoegh AM
FAU - Bzorek, Michael
AU  - Bzorek M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Biopsy
MH  - Female
MH  - Genotype
MH  - HLA Antigens/*genetics/*metabolism
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*genetics/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Oligonucleotide Array Sequence Analysis
MH  - Placenta/*metabolism
MH  - Polymorphism, Genetic/*genetics
MH  - Pre-Eclampsia/genetics
MH  - Pregnancy
MH  - Pregnancy Trimester, Third/genetics
MH  - RNA, Messenger/genetics/metabolism
EDAT- 2004/09/18 05:00
MHDA- 2005/03/05 09:00
CRDT- 2004/09/18 05:00
PHST- 2004/09/18 05:00 [pubmed]
PHST- 2005/03/05 09:00 [medline]
PHST- 2004/09/18 05:00 [entrez]
AID - AJI208 [pii]
AID - 10.1111/j.1600-0897.2004.00208.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2004 Sep;52(3):212-7. doi: 10.1111/j.1600-0897.2004.00208.x.