PMID- 15374714
OWN - NLM
STAT- MEDLINE
DCOM- 20041110
LR  - 20220317
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 82
IP  - 3
DP  - 2004 Sep
TI  - Quantitative assessment of ischemic tissue damage in ovarian cortical tissue with 
      or without antioxidant (ascorbic acid) treatment.
PG  - 679-85
AB  - OBJECTIVE: To estimate ischemic tissue damage in ovarian cortex and to evaluate 
      the effectiveness of ascorbic acid, an antioxidant, to protect ovarian tissue 
      from apoptosis caused by ischemia. DESIGN: In vitro laboratory experiments. 
      SETTING: Academic research institute. INTERVENTION(S): Fresh and frozen/thawed 
      cortical sections of bovine ovaries were incubated in MEM medium with or without 
      ascorbic acid for a duration of 3, 24, and 48 hours at 37 degrees C. MAIN OUTCOME 
      MEASURE(S): Oxygen consumption rates, lactate dehydrogenase concentrations, 
      apoptosis rates determined by terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL) staining, and DNA fragmentation analysis. RESULT(S): 
      The oxygen consumption rates were correlated inversely with the duration of 
      incubation. When the rates of apoptosis in primordial follicles with or without 
      ascorbic acid treatment were compared, there was no statistically significant 
      difference between the two groups. However, the ascorbic acid treatment group 
      showed significantly decreased apoptosis in ovarian cortex (stromal cells) with 
      24 hours of incubation. CONCLUSION(S): The correlation between ischemic tissue 
      damage and the duration of ischemia was verified. Ovarian cortex could tolerate 
      ischemia at least for 3 hours. Ascorbic acid treatment reduced apoptosis in 
      ovarian cortex up to 24 hours of incubation in vitro. It appeared that stromal 
      cells were more vulnerable to ischemia compared to primordial follicles.
FAU - Kim, S Samuel
AU  - Kim SS
AD  - Eulji Life Science Institute, Eulji University School of Medicine, Seoul, South 
      Korea. medssk@attglobal.net
FAU - Yang, Hyun Won
AU  - Yang HW
FAU - Kang, Hee Gyoo
AU  - Kang HG
FAU - Lee, Hang Heun
AU  - Lee HH
FAU - Lee, Hoi Chang
AU  - Lee HC
FAU - Ko, Duck Sung
AU  - Ko DS
FAU - Gosden, Roger G
AU  - Gosden RG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Antioxidants)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Apoptosis/drug effects
MH  - Ascorbic Acid/*pharmacology
MH  - Cattle
MH  - Disease Models, Animal
MH  - Female
MH  - Ischemia/*pathology
MH  - Ovary/*blood supply/drug effects/metabolism
MH  - Oxygen Consumption
EDAT- 2004/09/18 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/09/18 05:00
PHST- 2003/09/04 00:00 [received]
PHST- 2004/05/08 00:00 [revised]
PHST- 2004/05/08 00:00 [accepted]
PHST- 2004/09/18 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/09/18 05:00 [entrez]
AID - S0015-0282(04)00979-3 [pii]
AID - 10.1016/j.fertnstert.2004.05.022 [doi]
PST - ppublish
SO  - Fertil Steril. 2004 Sep;82(3):679-85. doi: 10.1016/j.fertnstert.2004.05.022.