PMID- 15376199
OWN - NLM
STAT- MEDLINE
DCOM- 20041222
LR  - 20061115
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 102
IP  - 5
DP  - 2004 Oct 25
TI  - Feasibility and utility of using chromosomal aneusomy to further define the 
      cytologic categories in nipple aspirate fluid specimens: a preliminary study.
PG  - 322-7
AB  - BACKGROUND: There is renewed interest in using the cytologic changes in the 
      epithelial cells obtained from specimens such as nipple aspiration fluid (NAF) 
      and ductal lavage for risk stratification of women at increased risk for 
      developing breast carcinoma. METHODS: Molecular tests such as fluorescence in 
      situ hybridization (FISH) have the potential to be used as adjuncts to 
      conventional cytology for more accurately categorizing cells in these types of 
      specimens. The current study investigated the feasibility and utility of FISH 
      analysis of aneusomy in chromosomes 1, 8, 11 and 17 as an adjunct to conventional 
      cytology in the classification of NAF specimens. RESULTS: The authors found 
      chromosomal aneusomy for at least one chromosome in all three malignant and both 
      markedly atypical cases. Of the five cases classified as being mildy atypical on 
      cytology, four were disomic, and only one showed aneusomy in chromosomes 8 and 
      11. CONCLUSIONS: The current study established the possibilities, limitations, 
      and feasibility of using FISH in conjunction with routine cytology for a more 
      accurate classification of ductal epithelial cells in NAF specimens. FISH-based 
      detection of chromosomal aneusomy helped to define mild atypia, thereby aiding in 
      the selection of the truly atypical cases for appropriate therapeutic 
      intervention. In addition, FISH-based detection of chromosomal aneusomy can also 
      be a valuable adjunct to conventional cytology in selected cases for confirming a 
      benign, suspicious, or malignant diagnosis.
FAU - Krishnamurthy, Savitri
AU  - Krishnamurthy S
AD  - Department of Cytopathology, Unit 53, The University of Texas M. D. Anderson 
      Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA. 
      skishna@mdanderson.org
FAU - Zhao, Lian
AU  - Zhao L
FAU - Hayes, Kimberly
AU  - Hayes K
FAU - Glassman, Armand B
AU  - Glassman AB
FAU - Cristofanilli, Massimo
AU  - Cristofanilli M
FAU - Singletary, S Eva
AU  - Singletary SE
FAU - Hunt, Kelly K
AU  - Hunt KK
FAU - Kuerer, Henry M
AU  - Kuerer HM
FAU - Sneige, Nour
AU  - Sneige N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Biopsy, Needle
MH  - Breast Neoplasms/*genetics/pathology
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Epithelial Cells/*pathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mammary Glands, Human/*cytology/*pathology
MH  - *Nipples/cytology/pathology
MH  - Retrospective Studies
EDAT- 2004/09/18 05:00
MHDA- 2004/12/23 09:00
CRDT- 2004/09/18 05:00
PHST- 2004/09/18 05:00 [pubmed]
PHST- 2004/12/23 09:00 [medline]
PHST- 2004/09/18 05:00 [entrez]
AID - 10.1002/cncr.20558 [doi]
PST - ppublish
SO  - Cancer. 2004 Oct 25;102(5):322-7. doi: 10.1002/cncr.20558.