PMID- 15377288
OWN - NLM
STAT- MEDLINE
DCOM- 20050331
LR  - 20171116
IS  - 0815-9319 (Print)
IS  - 0815-9319 (Linking)
VI  - 19
IP  - 10
DP  - 2004 Oct
TI  - Decreased expression of B7 costimulatory molecules and major histocompatibility 
      complex class-I in human hepatocellular carcinoma.
PG  - 1121-7
AB  - BACKGROUND AND AIM: We analyzed the expression of antigen-processing and 
      antigen-presenting molecules in surgically resected fresh samples of human 
      hepatocellular carcinoma (HCC) tissue to elucidate a mechanism of immune escape. 
      We also examined the expression of interleukin (IL)-10 protein, which might act 
      to downregulate expression of antigen-processing and antigen-presenting 
      molecules. METHODS: Twenty-eight HCC samples obtained by surgical resection were 
      analyzed for the expression of beta2-microglobulin, heat-shock protein (HSP)-70, 
      human leukocyte antigen (HLA) class-I, CD80 (B7-1), CD86 (B7-2) and IL-10 by 
      immunostaining. RESULTS: Beta2-microglobulin and HSP-70 were preserved in all 
      samples. In contrast, the expression of HLA class-I molecules was significantly 
      reduced according to lowering in the histological grading of tumor 
      differentiation (P = 0.024). Furthermore, B7-1 and B7-2 expression was reduced in 
      tumor cells compared with corresponding areas of liver tissue without malignant 
      involvement irrespective of the histological grading of tumors (21% and 36%, 
      respectively). Although IL-10 protein was expressed in 54% of HCC, no 
      relationship between the expression of IL-10 and downregulation of B7-1, B7-2, 
      and HLA class-I was evident. CONCLUSION: These findings suggest the potential 
      role of B7 co-stimulatory molecules and HLA class-I molecules in facilitating HCC 
      escape from immune surveillance without the involvement of IL-10.
FAU - Fujiwara, Keishi
AU  - Fujiwara K
AD  - Department of Medicine and Medical Science, Okayama University Graduate School of 
      Medicine and Dentistry, Okayama, Japan.
FAU - Higashi, Toshihiro
AU  - Higashi T
FAU - Nouso, Kazuhiro
AU  - Nouso K
FAU - Nakatsukasa, Harushige
AU  - Nakatsukasa H
FAU - Kobayashi, Yoshiyuki
AU  - Kobayashi Y
FAU - Uemura, Masayuki
AU  - Uemura M
FAU - Nakamura, Shin-Ichiro
AU  - Nakamura S
FAU - Sato, Shuichiro
AU  - Sato S
FAU - Hanafusa, Tadashi
AU  - Hanafusa T
FAU - Yumoto, Yasuhiro
AU  - Yumoto Y
FAU - Naito, Ichiro
AU  - Naito I
FAU - Shiratori, Yasushi
AU  - Shiratori Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - J Gastroenterol Hepatol
JT  - Journal of gastroenterology and hepatology
JID - 8607909
RN  - 0 (B7-1 Antigen)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (beta 2-Microglobulin)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Aged
MH  - B7-1 Antigen/*biosynthesis
MH  - Carcinoma, Hepatocellular/*immunology
MH  - Female
MH  - Genes, MHC Class I/*immunology
MH  - HSP70 Heat-Shock Proteins/biosynthesis/immunology
MH  - Humans
MH  - Interleukin-10/biosynthesis/immunology
MH  - Liver Neoplasms/*immunology
MH  - Male
MH  - beta 2-Microglobulin/biosynthesis/immunology
EDAT- 2004/09/21 05:00
MHDA- 2005/04/01 09:00
CRDT- 2004/09/21 05:00
PHST- 2004/09/21 05:00 [pubmed]
PHST- 2005/04/01 09:00 [medline]
PHST- 2004/09/21 05:00 [entrez]
AID - JGH3467 [pii]
AID - 10.1111/j.1440-1746.2004.03467.x [doi]
PST - ppublish
SO  - J Gastroenterol Hepatol. 2004 Oct;19(10):1121-7. doi: 
      10.1111/j.1440-1746.2004.03467.x.