PMID- 15377882
OWN - NLM
STAT- MEDLINE
DCOM- 20050420
LR  - 20181113
IS  - 0145-479X (Print)
IS  - 0145-479X (Linking)
VI  - 36
IP  - 4
DP  - 2004 Aug
TI  - Development and initial characterization of xenomitochondrial mice.
PG  - 421-7
AB  - Xenomitochondrial mice harboring trans-species mitochondria on a Mus musculus 
      domesticus (MD) nuclear background were produced. We created xenomitochondrial ES 
      cell cybrids by fusing Mus spretus (MS), Mus caroli (MC), Mus dunni (Mdu), or Mus 
      pahari (MP) mitochondrial donor cytoplasts and rhodamine 6-G treated CC9.3.1 or 
      PC4 ES cells. The selected donor backgrounds reflected increasing evolutionary 
      divergence from MD mice and the resultant mitochondrial-nuclear mismatch targeted 
      a graded respiratory chain defect. Homoplasmic (MS, MC, Mdu, and MP) and 
      heteroplasmic (MC) cell lines were injected into MD ova, and liveborn chimeric 
      mice were obtained (MS/MD 18 of 87, MC/MD 6 of 46, Mdu/MD 31 of 140, and MP/MD l 
      of 9 founder chimeras, respectively). Seven MS/MD, 1 MC/MD, and 11 Mdu/MD 
      chimeric founder females were mated with wild-type MD males, and 18 of 19 (95%) 
      were fertile. Of fertile females, only one chimeric MS/MD (1% coat color 
      chimerism) and four chimeric Mdu/MD females (80-90% coat color chimerism) 
      produced homoplasmic offspring with low efficiency (7 of 135; 5%). Four male and 
      three female offspring were homoplasmic for the introduced mitochondrial 
      backgrounds. Three male and one female offspring proved viable. Generation of 
      mouse lines using additional female ES cell lineages is underway. We hypothesize 
      that these mice, when crossbred with neurodegenerative-disease mouse models, will 
      show accelerated age-related neuronal loss, because of their suboptimal capacity 
      for oxidative phosphorylation and putatively increased oxidative stress.
FAU - Trounce, I A
AU  - Trounce IA
AD  - Genomic Disorders Research Centre, and Centre for Neuroscience, University of 
      Melbourne, Victoria, Australia.
FAU - McKenzie, M
AU  - McKenzie M
FAU - Cassar, C A
AU  - Cassar CA
FAU - Ingraham, C A
AU  - Ingraham CA
FAU - Lerner, C A
AU  - Lerner CA
FAU - Dunn, D A
AU  - Dunn DA
FAU - Donegan, C L
AU  - Donegan CL
FAU - Takeda, K
AU  - Takeda K
FAU - Pogozelski, W K
AU  - Pogozelski WK
FAU - Howell, R L
AU  - Howell RL
FAU - Pinkert, C A
AU  - Pinkert CA
LA  - eng
GR  - DE12634/DE/NIDCR NIH HHS/United States
GR  - RR16286/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Bioenerg Biomembr
JT  - Journal of bioenergetics and biomembranes
JID - 7701859
RN  - 0 (DNA, Mitochondrial)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - DNA, Mitochondrial/*genetics
MH  - *Disease Models, Animal
MH  - Female
MH  - Genetic Engineering/*methods
MH  - Hybridization, Genetic/genetics
MH  - Male
MH  - Mice
MH  - Mice, Transgenic/*genetics
MH  - Mitochondria/*genetics
MH  - Mitochondrial Diseases/*genetics
MH  - Neurodegenerative Diseases/*genetics
EDAT- 2004/09/21 05:00
MHDA- 2005/04/21 09:00
CRDT- 2004/09/21 05:00
PHST- 2004/09/21 05:00 [pubmed]
PHST- 2005/04/21 09:00 [medline]
PHST- 2004/09/21 05:00 [entrez]
AID - 490865 [pii]
AID - 10.1023/B:JOBB.0000041778.84464.16 [doi]
PST - ppublish
SO  - J Bioenerg Biomembr. 2004 Aug;36(4):421-7. doi: 
      10.1023/B:JOBB.0000041778.84464.16.