PMID- 15378006
OWN - NLM
STAT- MEDLINE
DCOM- 20041206
LR  - 20201208
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 23
IP  - 50
DP  - 2004 Oct 28
TI  - The FHA domain protein SNIP1 is a regulator of the cell cycle and cyclin D1 
      expression.
PG  - 8185-95
AB  - Smad nuclear interacting protein 1 (SNIP1) is an evolutionarily conserved protein 
      containing a forkhead-associated (FHA) domain that regulates gene expression 
      through interactions with multiple transcriptional regulators. Here, we have used 
      short interfering RNAs (siRNAs) to knockdown SNIP1 expression in human cell 
      lines. Surprisingly, we found that reduction in SNIP1 levels resulted in 
      significantly reduced cell proliferation and accumulation of cells in the G1 
      phase of the cell cycle. Consistent with this result, we observed that cyclin D1 
      protein and mRNA levels were reduced. Moreover, SNIP1 depletion results in 
      inhibition of cyclin D1 promoter activity in a manner dependent upon a previously 
      characterized binding site for the AP-1 transcription factor family. SNIP1 itself 
      is induced upon serum stimulation immediately prior to cyclin D1 expression. 
      These effects were independent of the tumour suppressors p53 and retinoblastoma 
      (Rb), but were consistent with an interaction with BRG1, a component of the 
      ATP-dependent chromatin remodelling complex, Swi/Snf. These results define both a 
      new function for SNIP1 and identify a previously unrecognized regulator of the 
      cell cycle and cyclin D1 expression.
FAU - Roche, Kevin C
AU  - Roche KC
AD  - Division of Gene Regulation and Expression, School of Life Sciences, MSI/WTB 
      Complex, Dow Street, University of Dundee, Dundee, DD1 5EH, UK.
FAU - Wiechens, Nicola
AU  - Wiechens N
FAU - Owen-Hughes, Tom
AU  - Owen-Hughes T
FAU - Perkins, Neil D
AU  - Perkins ND
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA Primers)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (SNIP1 protein, human)
RN  - 0 (Trans-Activators)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Base Sequence
MH  - Cell Division/*physiology
MH  - Cyclin D1/genetics/*metabolism
MH  - DNA Primers
MH  - Gene Expression Regulation/physiology
MH  - HeLa Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/*physiology
MH  - Nuclear Proteins/metabolism
MH  - Promoter Regions, Genetic
MH  - RNA, Small Interfering
MH  - RNA-Binding Proteins
MH  - Trans-Activators/metabolism
EDAT- 2004/09/21 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/09/21 05:00
PHST- 2004/09/21 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/09/21 05:00 [entrez]
AID - 1208025 [pii]
AID - 10.1038/sj.onc.1208025 [doi]
PST - ppublish
SO  - Oncogene. 2004 Oct 28;23(50):8185-95. doi: 10.1038/sj.onc.1208025.