PMID- 1537907
OWN - NLM
STAT- MEDLINE
DCOM- 19920330
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 30
IP  - 2
DP  - 1992 Feb
TI  - Molecular epidemiology of Haemophilus influenzae type b in the Gambia.
PG  - 386-90
AB  - One hundred two invasive and 64 noninvasive isolates of Haemophilus influenzae 
      were collected in the course of a 2-year prospective field study on the 
      epidemiology of H. influenzae meningitis in The Gambia. The isolates were 
      serotyped, biotyped, and subtyped by outer membrane protein (OMP) profile 
      analysis (OMP subtyping). H. influenzae meningitis was found to be caused by 
      serotype b (95%). In invasive disease, serotype a, although present in the throat 
      of healthy children, caused only occasionally (5.9%) disease. The distribution of 
      biotypes of H. influenzae appeared to be very similar to that found outside The 
      Gambia. A distinct pattern of OMP subtypes, different from other parts of the 
      world, is prevalent in H. influenzae type b (Hib) in The Gambia. OMP subtypes 2, 
      4, 5, 8, and 9 were observed to be predominant. These subtypes, except subtype 2, 
      have not been described. L subtypes (subtypes 2, 4, and 8) were associated with 
      invasive disease, whereas non-L subtypes (subtypes 5 and 9) were found more often 
      in healthy carriers (P less than 0.001). A significant difference in geographical 
      distribution was found in subtypes of noninvasive Hib strains (P less than 0.05). 
      We conclude that in The Gambia H. influenzae invasive disease is caused mainly by 
      type b strains with a limited number of OMP subtypes, which are different from 
      the subtypes found elsewhere in the world. These data are important for the 
      surveillance of Hib disease in developing countries and are baseline data for a 
      Hib polyribosyl-ribitolphosphate-conjugated vaccine trial in The Gambia. 
      Alternative Hib OMP vaccines should include a set of representative OMPs.
FAU - Bijlmer, H A
AU  - Bijlmer HA
AD  - Medical Research Council Laboratories, Fajara, The Gambia.
FAU - van Alphen, L
AU  - van Alphen L
FAU - Geelen-van den Broek, L
AU  - Geelen-van den Broek L
FAU - Greenwood, B M
AU  - Greenwood BM
FAU - Valkenburg, H A
AU  - Valkenburg HA
FAU - Dankert, J
AU  - Dankert J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Haemophilus Vaccines)
RN  - 0 (Haemophilus influenzae type b polysaccharide vaccine)
RN  - 0 (Polysaccharides, Bacterial)
SB  - IM
MH  - Bacterial Capsules
MH  - Bacterial Outer Membrane Proteins/analysis
MH  - Bacterial Typing Techniques
MH  - Bacterial Vaccines/therapeutic use
MH  - Epidemiologic Methods
MH  - Gambia/epidemiology
MH  - Haemophilus Infections/*epidemiology/microbiology/prevention & control
MH  - *Haemophilus Vaccines
MH  - *Haemophilus influenzae/chemistry/classification/isolation & purification
MH  - Humans
MH  - Polysaccharides, Bacterial/therapeutic use
MH  - Serotyping
PMC - PMC265065
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
PMCR- 1992/02/01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
PHST- 1992/02/01 00:00 [pmc-release]
AID - 10.1128/jcm.30.2.386-390.1992 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1992 Feb;30(2):386-90. doi: 10.1128/jcm.30.2.386-390.1992.