PMID- 15380442
OWN - NLM
STAT- MEDLINE
DCOM- 20050124
LR  - 20061115
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 176
IP  - 2
DP  - 2004 Oct
TI  - Apolipoprotein B-48: comparison of fasting concentrations measured in 
      normolipidaemic individuals using SDS-PAGE, immunoblotting and ELISA.
PG  - 207-17
AB  - Raised levels of chylomicrons and chylomicron remnants, which circulate following 
      a meal, have been implicated in the development of atherosclerosis. 
      Apolipoprotein (apo) B-48 is exclusively associated with chylomicron particles 
      and provides a specific direct measurement of the number of intestinally derived 
      lipoproteins in the circulation. The quantification of apo B-48 in biological 
      samples is difficult due to the very low concentration in plasma, structural 
      similarity to the N-terminal 48% of apo B-100 and lack of an appropriate standard 
      for apo B-48. Sodium dodecyl sulphate polyacrylamide gel electrophoresis 
      (SDS-PAGE), followed by coomassie blue staining, has been used for many years to 
      measure apo B-48 levels in triacylglycerol (TAG)-rich lipoprotein samples. The 
      raising of antiserum to apo B-48 has led to development of more sensitive and 
      specific methods including immunoblotting and enzyme-linked immunosorbant assays 
      (ELISAs). This has enabled direct measurement of apo B-48 in plasma without the 
      need for separation into TAG-rich lipoproteins. A high degree of variability was 
      observed in the apo B-48 concentrations reported in the literature both within 
      and between the SDS-PAGE, immunoblotting and ELISA methods.
FAU - Jackson, Kim G
AU  - Jackson KG
AD  - Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, The University 
      of Reading, Reading, Berkshire, UK. k.g.jackson@reading.ac.uk
FAU - Williams, Christine M
AU  - Williams CM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Review
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (Apolipoprotein B-48)
RN  - 0 (Apolipoproteins B)
RN  - 0 (Lipids)
SB  - IM
MH  - Apolipoprotein B-48
MH  - Apolipoproteins B/*blood
MH  - Arteriosclerosis/*physiopathology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Fasting
MH  - Humans
MH  - Hyperlipidemias
MH  - Immunoblotting
MH  - Lipids/blood
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
RF  - 51
EDAT- 2004/09/24 05:00
MHDA- 2005/01/26 09:00
CRDT- 2004/09/24 05:00
PHST- 2003/10/13 00:00 [received]
PHST- 2004/01/26 00:00 [revised]
PHST- 2004/02/05 00:00 [accepted]
PHST- 2004/09/24 05:00 [pubmed]
PHST- 2005/01/26 09:00 [medline]
PHST- 2004/09/24 05:00 [entrez]
AID - S0021-9150(04)00081-4 [pii]
AID - 10.1016/j.atherosclerosis.2004.02.003 [doi]
PST - ppublish
SO  - Atherosclerosis. 2004 Oct;176(2):207-17. doi: 
      10.1016/j.atherosclerosis.2004.02.003.